原苏木素B抗结肠癌细胞的作用及机制  

effect and mechanism of protohematoxylin B on colon cancer cells

作　　者：金少雄 邱成志[2] 林清江[1] 曾荣耀 杨锦锋 许泽波 JIN Shao-xiong;QIU Cheng-zhi;LIN Qing-jiang;无(Emergency Department of the Second Affiliated Hospital of Fujian Medical University,Quanzhou 362000,China;Department of General Surgery,The Second Affiliated Hospital of Fujian Medical University,Quanzhou 362000,China)

机构地区：[1]福建医科大学附属第二医院急诊科,福建泉州362000 [2]福建医科大学附属第二医院普外科,福建泉州362000

出　　处：《吉林医学》2023年第8期2067-2071,共5页Jilin Medical Journal

基　　金：福建省中医药科技项目[项目编号:2021zyyj73]。

摘　　要：目的:研究原苏木素B(PSB)对人结肠癌SW620、SW480细胞的增殖、迁移、侵袭及凋亡的影响,并探讨其抗结肠癌细胞的作用及机制。方法:实验1:将SW620、SW480细胞各分为7组,分别为对照组(不加药物经DMSO处理)和PSB-1~PSB-6组(分别给予6.25μg/ml、12.50μg/ml、25.00μg/ml、50.00μg/ml、100.00μg/ml和200.00μg/ml PSB),并分别处理24 h、48 h、72 h和96 h。实验2:将结肠癌SW620、SW480细胞各分为4组:对照组(不加药物经DMSO处理)、PSB-7~PSB-9组(分别给予17.5μg/ml、35.0μg/ml和70.0μg/ml PSB处理24 h);另外SW620细胞中又分为实验1组(25.0 ng/ml IGF-1处理24 h)、实验2组(25.0 ng/ml IGF-1+35.0μg/ml PSB处理24 h)、实验3组(1μmol/L AZD2858处理24 h)和实验4组(1μmol/L AZD2858+35.0μg/ml PSB处理24 h)。检测SW620、SW480细胞增殖、迁移、侵袭、凋亡情况、高尔基磷蛋白3(GOLPH3)及SW620细胞磷酸化蛋白激酶B(p-AKT)、磷酸化p70核糖体蛋白S6激酶(p-p70S6K)、β-连环蛋白(β-catenin)的表达水平。结果:PSB以浓度和时间依赖效应抑制SW620、SW480细胞的增殖。在SW620细胞中,PSB-8组与对照组的细胞迁移数目分别为(367.67±8.18)个和(890.00±19.65)个,细胞侵袭数目分别为(122.33±9.18)个和(374.67±13.47)个,细胞凋亡率分别为(27.74±2.57)%和(6.13±0.45)%,PSB-7~PSB-9组和对照组的GOLPH3蛋白相对表达量分别为(0.52±0.03)、(0.36±0.03)、(0.07±0.01)和(0.96±0.12),PSB-8组、对照组及实验2组中的p-AKT的蛋白相对表达量分别为(0.10±0.05)、(1.04±0.34)和(10.00±1.61),p-p70S6K的蛋白相对表达量分别为(0.40±0.14)、(1.05±0.12)和(2.52±0.29),β-catenin的蛋白相对表达量分别为(0.14±0.07)、(0.99±0.23)、(6.06±0.89),对照组、PSB-8组、实验3组和实验4组的相对荧光强度值分别为(1.00±0.07)、(0.14±0.06)、(1.98±0.16)和(1.31±0.14),差异均有统计学意义(均P<0.001);在SW480细胞中,PSB-8组与对照组的细胞迁移数目分别为(314.33±5.31)个和(589.67±22.87)个Objective To study the effect of pro-hematoxylin B(PSB)on the proliferation,migration,invasion,and apoptosis of human colon cancer SW620 and SW480 cells,and to explore its anti-colon cancer cell effect and mechanism.Method Experiment 1:SW620 and SW480 cells were divided into 7 groups,namely the control group(treated with DMSO without drugs)and the PSB-1~PSB-6 groups(treated with 6.25,12.50,25.00,50.00,100.00 and 200.00μg/ml PSB,respectively)and treated for 24,48,72,and 96 hours,respectively;Experiment 2:Colon cancer SW620 and SW480 cells were divided into four groups:control group(treated with DMSO without drugs),PSB-7-PSB-9 group(treated with 17.5,35.0,and 70.0μg/ml PSB treatment for 24 hours,respectively);In addition,SW620 cells were divided into experimental group 1(25.0 ng/ml IGF-1 treatment for 24 hours)and experimental group 2(25.0 ng/ml IGF-1+35.0μg/ml PSB treatment for 24 hours),experimental group 3(1μmol/L AZD2858 treatment for 24 hours and experimental group 4(1μmol/L AZD2858+35.0μg/ml PSB treatment for 24 hours).Detection of cell proliferation,migration,invasion,apoptosis,and Golgi phosphoprotein 3(GOLPH3),phosphorylated protein kinase B(p-AKT),p70 ribosomal protein s6 kinase(p-p70S6K),β-catenin(β-catenin)expression.Results PSB inhibited the proliferation of SW620 and SW480 cells in a concentration and time-dependent manner.In SW620 cells,the number of cell migration was(367.67±8.18)and(890.00±19.65)in psb-8 group and control group,the number of cell invasion was(122.33±9.18)and(374.67±13.47),the apoptosis rate was(27.74±2.57)%and(6.13±0.45)%respectively,and the relative expression of GOLPH3 protein in PSB-7~PSB-9 group and control group was(0.52±0.03),(0.36±0.03),(0.07±0.01)and(0.96±0.12),respectively,The relative protein expression of p-Akt in PSB-8 group,control group and experimental group 2 was(0.10±0.05),(1.04±0.34)and(10.00±1.61)respectively,and the relative protein expression of p-p70s6k was(0.40±0.14),(1.05±0.12)and(2.52±0.29)respectively,the relative expression levels

关 键 词：原苏木素B 高尔基磷蛋白3 结肠癌 磷脂酰肌醇3激酶/蛋白激酶B/哺乳动物西罗莫司靶蛋白 Wnt家族分泌蛋白/β-连环蛋白 

分 类 号：R735.35[医药卫生—肿瘤]