基质金属蛋白酶-9基因的多态性与缺血性脑血管病颈动脉粥样硬化不稳定斑块相关性研究  

Correlation between matrix metalloproteinase-9 gene polymorphism and unstable plaque formation in carotid atherosclerosis

作　　者：沈焱[1] 李伟 刘志梅 张启帆 许瑞庭[3] SHEN Yan;LI Wei;LIU Zhimei;ZHANG Qifan;XU Ruiting(Neurology Department,People′s Hospital of Ningxia Hui Autonomous Region,Yinchuan 750002,China;Rheumatology and Immunology Department,People′s Hospital of Ningxia Hui Autonomous Region,Yinchuan 750002,China;Breast Surgery,People′s Hospital of Ningxia Hui Autonomous Region,Yinchuan 750002,China)

机构地区：[1]宁夏回族自治区人民医院神经内科,宁夏银川750002 [2]宁夏回族自治区人民医院风湿免疫科,宁夏银川750002 [3]宁夏回族自治区人民医院甲乳外科,宁夏银川750002

出　　处：《宁夏医学杂志》2023年第7期585-588,I0001,共5页Ningxia Medical Journal

摘　　要：目的探讨基质金属蛋白酶-9基因(MMP-9)启动子C-1562T多态性与脑血管病颈动脉粥样硬化不稳定斑块相关性。方法对缺血性脑血管病患者进行颈动脉超声的检查,筛选伴有颈动脉粥样硬化不稳定斑块患者120例,设立健康对照组(100例),记录临床资料,并采用聚合酶链反应—限制性片段长度多态性(PCR-RFLP)方法对入选患者的MMP-9基因启动子C1562T位点进行基因检测,统计两组基因型、等位基因频率,并分析基因多态性是否与动脉粥样硬化不稳定斑块的形成存在相关性。结果缺血性脑血管病患者的年龄及疾病相关危险因素,包括吸烟、饮酒、血糖、血脂、血压、超敏CRP、同型半胱氨酸等均与动脉粥样硬化的不稳定斑块形成相关(P<0.05);病例组CT、TT基因型和T等位基因的频率与对照组相比显著增高(P<0.05);基质金属蛋白酶-9的T等位基因可能是不稳定斑块形成的危险因素(P<0.05)。结论缺血性脑血管病的相关危险因素与脑血管疾病的颈动脉粥样硬化不稳定斑块形成存在相关性;基质金属蛋白酶-9基因C-1562T多态性与动脉粥样硬化不稳定斑块的形成存在相关性;T等位基因可能是动脉粥样硬化不稳定斑块形成的易感基因。Objective To investigate the relationship between polymorphism at promoter of C1562T of matrix metalloproteinases-9(MMP-9)with unstable plaque formation of carotid atherosclerotic.Methods Carotid artery ultrasound examination was performed on patients diagnosed with ischemic stroke,100 patients with carotid atherosclerotic unstable plaque were selected as the case group,and 50 healthy control group were set up.The clinical data were recorded and the MMP-9 promoter C1562T locus was genotyped by polymerase chain reaction-restriction fragment length polymorphism(PCR_RFLP)method.The genotype and allele frequency of the two groups of patients were analyzed,and whether the polymorphism of MMP-9 gene promoter C-1562T was correlated with the formation of carotid atherosclerotic unstable plaque was analyzed.Results Risk factors of ischemic encephalopathy including its age,smoking,alcohol intake,blood sugar,blood fat,blood pressure,super sensitive CRP,homocysteine had relation with formation of unstable plaques of carotid atherosclerotic(P<0.05).The case group CT type,TT genotype and T allele frequency were significantly higher than those in control group(P<0.05).The T allele of MMP-9 was dangerous factors of carotid atherosclerotic who had unstable plaque(P<0.05).Conclusion Some risk factors of ischemic encephalopathy have correlation with unstable plaque formation of carotid atherosclerotic;polymorphism of MMP-9 gene C-1562 T have correlation with unstable plaque formation of carotid atherosclerotic.T allele may be susceptibility genes with unstable plaque formation of carotid atherosclerotic.

关 键 词：基质金属蛋白酶-9 缺血性脑血管病 动脉粥样硬化不稳定斑块 

分 类 号：R743[医药卫生—神经病学与精神病学]