施加外源抗坏血酸促进马铃薯试管薯形成及其机制初步研究  

A Preliminary Study on Promotional Effect of Application of Exogenous Ascorbic Acid on the Formation of in vitro Tubers of Potato and Its Possible Mechanism

作　　者：雷春霞 叶明旺 李灿辉[2] 龚明[1] LEI Chunxia;YE Mingwang;LI Canhui;GONG Ming(School of Life Sciences,Yunnan Normal University,Key Laboratory of Potato Biology of Yunnan Province,Engineering Research Center of Sustainable Development and Utilization of Biomass Energy of Ministry of Education,Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province,Kunming 650500,China;Joint Academy of Potato Sciences,Yunnan Normal University,Key Laboratory of Potato Biology of Yunnan Province,Kunming 650500,China)

机构地区：[1]云南师范大学生命科学学院,云南省马铃薯生物学重点实验室,生物能源持续开发利用教育部工程研究中心,云南省生物质能与环境生物技术重点实验室,昆明650500 [2]云南师范大学马铃薯科学研究院,云南省马铃薯生物学重点实验室,昆明650500

出　　处：《西北植物学报》2023年第6期956-967,共12页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(32260459);云南省马铃薯生物学重点实验室开放基金项目(YNPKF202202)。

摘　　要：为研究抗坏血酸(AsA)处理对马铃薯试管薯形成和结薯相关基因表达的影响及敲除结薯关键基因Solanum tuberosum self-prunning 6A(StSP6A)对AsA诱导马铃薯结薯的效应,利用不同浓度(0、1、5、10、20和50 mmol/L)外源AsA处理2个二倍体马铃薯CIP-149(Solanum phureja)、CIP-178(S.ajanhuiri)和四倍体C-88(S.tuberosum)。结果表明,1~5 mmol/L外源AsA处理可显著诱导马铃薯块茎的形成。对10个块茎形成相关基因的表达分析结果表明,外源添加1 mmol/L AsA可显著影响块茎形成相关基因的表达,与对照相比,总体上呈正调控基因表达增强或负调控因子表达被抑制的趋势,特别是StSP6A在AsA处理过程中的块茎形成早期表达量极显著上调,敲除StSP6A可消除外源AsA对马铃薯块茎形成的诱导作用。这些结果表明,AsA诱导马铃薯块茎形成是通过调控与块茎形成相关的基因表达来实现的,而StSP6A在AsA诱导马铃薯块茎形成中起关键作用。In the present study,the effects of exogenous ascorbic acid(AsA)treatments on potato microtuber formation and the expression of tuberization-related genes,as well as the influence of knockout of the key tuberization-related gene Solanum tuberosum self-prunning 6A(StSP6A)on the AsA-induced tuberization were investigated.Two diploid landraces CIP-149(Solanum phureja),CIP-178(S.ajanhuiri)and tetraploid C-88(S.tuberosum)were treated with different concentrations(0,1,5,10,20,50 mmol/L)of AsA.The results showed that exogenous AsA treatments with 1 mmol/L to 5 mmol/L could significantly induce tuber formation.The expression analysis of 10 tuberization-related genes demonstrated that the exogenous 1 mmol/L AsA treatment also significantly affected the expression of those tuberization-related genes,in which a general trend was demonstrated with an enhancement of positive regulator or repression of negative regulator expression for tuber formation as compared with the control,especially StSP6A,which was extremely significantly up-regulated during the early stage of tuber formation and AsA treatment.Knockout of StSP6A resulted in elimination of the effect of exogenous AsA-induced tuberizations in potato.These findings indicated that AsA-induced tuber formation is triggered by regulating the tuberization-related gene expression,and StSP6A played key roles in AsA-induced tuber formation in potato.

关 键 词：马铃薯 块茎形成 抗坏血酸(AsA) 结薯相关基因 StSP6A敲除 

分 类 号：Q785[生物学—分子生物学] Q786Q789