响应面法优化纤维素降解菌的培养条件和酶活力测定条件  

Optimization of culture conditions and enzyme activity determination conditions of cellulose degrading bacteria by response surface methodology

作　　者：刘夏 余金明 李晓悦 倪伟[1] LIU Xia;YU Jin-ming;LI Xiao-yue;NI Wei

机构地区：[1]石河子大学生命科学学院,新疆石河子832000

出　　处：《饲料研究》2023年第14期50-55,共6页Feed Research

基　　金：兵团科技计划项目(项目编号:2021CB033)。

摘　　要：试验旨在优化纤维素降解菌CE41的培养条件和纤维素酶酶促反应的条件,实现菌株的高产和纤维素酶的高效酶促反应。通过单因素试验和响应面法得出CE41的最佳培养条件为温度最适值38.67℃、pH值6.93、接种量6.14mL,此时酶活力最高,为27.556U/mL;酶促反应的最佳条件为反应温度58.19℃、pH值5.09、CMC-Na浓度1.42%,此时酶活力最高,为28.402U/mL。研究表明,试验结果可为后续研究奠定基础。The aim of the study was to optimize the culture conditions and cellulase enzymatic reaction conditions of the cellulase-degrading bacterium CE4l to achieve high yield and high efficiency cellulase enzymatic reaction of the strain.According to the results of single factor experiment and response surface method,the optimum culture conditions of CE41 were as follows:optimum temperature of 38.67 C,pH value of 6.93,inoculation amount of 6.14 mL,and the maximum enzyme activity was 27.556 U/mL.The optimum conditions for enzymatic reaction were as follows:optimum reaction temperature of 58.19℃C,pH value of 5.09,CMC-Na concentration of 1.42%,and the maximum enzyme activity of 28.402 U/mL.The study indicates that the experimental results can lay a foundation for subsequent research.

关 键 词：纤维素降解菌 纤维素 纤维素酶 响应面法 

分 类 号：S816.7[农业科学—饲料科学]