Nanostructured biohybrid material with wide-ranging antiviral action  

作　　者：Noelia Losada-Garcia Angela Vazquez-Calvo David Ortega-Alarcon Olga Abian Adrian Velazquez-Campoy Pilar Domingo-Calap Antonio Alcami Jose M.Palomo 

机构地区：[1]Instituto de Catálisis y Petroleoquímica(ICP),CSIC,C/Marie Curie 2.Madrid 28049,Spain [2]Centro de Biología Molecular Severo Ochoa,Consejo Superior de Investigaciones Científicas(CSIC)-Universidad Autónoma de Madrid(UAM),Madrid 28049,Spain [3]Institute of Biocomputation and Physics of Complex Systems(BIFI),Joint Unit GBsC-CSIC-BIFI,University of Zaragoza,Zaragoza 50018,Spain [4]Fundación Instituto de Investigación Sanitaria de Aragón(IIS Aragón),Zaragoza 50009,Spain [5]CIBERehd,Madrid 28029,Spain [6]Instituto de Biología Integrativa de Sistemas,I2SyBio,Universitat de València-CSIC,València E-46071,Spain

出　　处：《Nano Research》2023年第8期11455-11463,共9页纳米研究（英文版）

基　　金：supported by the Spanish National Research Council(CSIC)(projects Intramural Grant 202020E254);CSIC PTI-Global Health SGL2103036(J.M.P);European Union(Nextgeneration EU).Authors thank Dr.Martinez from Novozymes;was supported by Fundación hna(A.V-C.and O.A.).P.D-C.was financially supported by a Ramón y Cajal contract RYC2019-028015-I funded by MCIN/AEI.

摘　　要：Respiratory pathogens kill more people than any other infectious agent each year worldwide.Development of novel,economically friendly,sustainable,and highly efficient materials against viruses is a major challenge.Herein,we describe a nanostructured material composed of very small crystalline phosphate copper nanoparticles synthesized using a new biohybrid technology that employs a biological agent for its formation at room temperature in aqueous media.The evaluation of different enzymes in the final preparation of the nanomaterial or even in synthetic methods was performed.Biochemical characterization revealed the formation of Cu species in the protein network.The best biomaterial synthesized using a lipase called BioCuNPs showed excellent inhibition capacity against functional proteins of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2);for example,assent 3-chymotrypsin like protease(3CLpro)complete inhibition was achieved by using 5µg/mL,or acetone(ACE)–spike protein interaction was inhibited by more than 80%in the presence of 400µg/mL of BioCuNPs.Taking these in vitro results into account,an efficacy analysis against human coronavirus 229E(HCoV-E229)coronavirus was performed.A virus reduction of 99%was obtained in 5 min.Additionally,SARS-CoV-2 virus was tested to demonstrate high efficiency,with>99%inhibition in 15 min using 500 microgram of material.To determine the wide applicability of this nanohybrid against viruses,an evaluation was carried out against a non-enveloped virus such as Human Rhinovirus(HRV-14),obtaining a virus reduction of 99.9%in 5 min.Finally,the virucidal capacity against different bacteriophages was also evaluated,obtaining an excellent inhibition effect against PhageΦX174(99.999%reduction in 5 min).

关 键 词：antiviral compound CORONAVIRUSES copper nanoparticles NANOBIOTECHNOLOGY viruses 

分 类 号：TB383[一般工业技术—材料科学与工程]