氧化锌/凹凸棒石纳米复合材料的抗菌性能和机制  

The antibacterial properties and mechanisms of ZnO/attapulgite nanocomposites

作　　者：马梦婷 宋亚萌 张弘 杨芳芳 刘欣跃[1] MA Meng-ting;SONG Ya-meng;ZHANG Hong;YANG Fang-fang;LIU Xin-yue(Laboratory Medicine Center,Lanzhou University Second Hospital,Lanzhou 730030,China;Key Laboratory of Clay Mineral Applied Research of Gansu Province,Center of Eco-materials and Green Chemistry,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China)

机构地区：[1]兰州大学第二医院检验医学中心,兰州730030 [2]中国科学院兰州化学物理研究所生态材料与绿色化学中心,甘肃省黏土矿物应用研究重点实验室,兰州730000

出　　处：《兰州大学学报（自然科学版）》2023年第3期398-405,414,共9页Journal of Lanzhou University(Natural Sciences)

基　　金：兰州大学第二医院博士研究生培养专项基金(YJS-BD-11)。

摘　　要：通过油橄榄叶介导合成氧化锌纳米粒子,将其负载于凹凸棒石上得到复合抗菌材料(ZnO/APT).通过扫描电子显微镜、透射电子显微镜等表征ZnO/APT的合成,通过琼脂稀释法研究ZnO/APT的抗菌活性.结果表明,ZnO/APT对金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌的最小抑菌浓度(MIC)分别为0.25和1.00mg/mL,对大肠埃希菌及产超广谱β内酰胺酶大肠埃希菌的MIC均为2.50mg/mL,对铜绿假单胞菌的MIC为1.00mg/mL,具有广谱抗菌性能.以ZnO/APT为抗菌剂,以大肠埃希菌为模型,通过透射电子显微镜、活/死细菌染色、活性氧检测、DNA琼脂糖凝胶电泳等手段研究其可能的抗菌机制.ZnO/APT纳米复合材料与细菌相互作用时能够捕获细菌,并由外部破坏细菌结构,由内部催化细菌活性氧含量,导致氧化应激失衡.ZnO/APT的接触加剧细菌生理活动的紊乱,抑制并损伤细菌DNA,使细菌死亡.The nanocomposite zinc oxide/attapulgite(ZnO/APT)was synthesized through the mediation of olive leaves,and the successful synthesis of the composite material was proved by scanning electron microscopy,transmission electron microscopy(TEM)and other characterizations.The minimum inhibi-tory concentration(MIC)of ZnO/APT was studied by the Agar dilution method.The results showed that the MIC of ZnO/APT against Staphylococcus aureus was 0.25 mg/mL,against methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa was 1.00 mg/mL,and against Escherichia coli and Escherichia coli producing extended-spectrumβ-lactamases was 2.50 mg/mL,respectively,which proved that the antibacterial properties were broad-spectrum.The antibacterial mechanism of ZnO/APT was ex-plored by TEM,live/dead bacteria staining,the level of reactive oxygen species(ROS)and DNA damage,using E.coli as model.The results showed that ZnO/APT captured bacteria from the outside and destroyed the bacterial structures.ROS content was increased from inside bacteria to aggravate oxidative stress response,and the direct effect of ZnO/APT and changes in bacterial oxidative stress response aggravated the disturbance in the physiological activities and DNA injury and inhibition,leading to damage ofbacterial cellsordeath.

关 键 词：氧化锌/凹凸棒石 广谱抗菌活性 抗菌机制 

分 类 号：R318.08[医药卫生—生物医学工程]