大蒜素调控RANK表达对破骨细胞分化成熟的影响  

Effects of Allicin Regulating RANK Expression on Osteoclast Differentiation and Maturation

作　　者：刘亚非[1] 闫桂艳[2] 田松波[3] 段程锦 李宪起 LIU Ya-fei;YAN Gui-yan;TIAN Song-bo;DUAN Cheng-jin;LI Xian-qi(Department of Orthodontics,The First Hospital of Hebei Medical University,Shjiazhuang 050300;Department of Stomatology,Hebei People's Hospital,Shijiazhuang 050057;Department of Stomatology,Second Hospital of Hebei Medical University,Shijiazhuang 050051;Shijiazhuang Qiaoxi Dental Hospital,Shijiazhuang 050024;Institute of Comprehensive Dental Medicine,Matsumoto Dental University,Nagano 399-0712)

机构地区：[1]河北医科大学第一医院口腔正畸科,石家庄050030 [2]河北省人民医院口腔科,石家庄050057 [3]河北医科大学第二医院口腔内科,石家庄050051 [4]石家庄市桥西口腔医院,石家庄050000 [5]日本松本齿科大学综合齿科医学研究所,日本长野399-0712

出　　处：《中国中西医结合杂志》2023年第7期851-856,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：河北省中医药管理局科研项目(No.2021123)。

摘　　要：目的 研究大蒜素调控核因子κB受体活化因子(RANK)对破骨细胞分化成熟的影响。方法 诱导小鼠骨髓间充质干细胞(BMSCs)分化破骨细胞,分为对照组及实验组,两组采用30 ng/mL巨噬细胞集落刺激因子(M-CSF)及60 ng/mL RANKL培养,实验组第1、3、7天进行大蒜素(20μg/mL)干预。采用CCK-8检测两组细胞的增殖情况,通过抗酒石酸酸性磷酸酶(TRAP)染色及定量检测TRAP酶活性,通过RT-PCR检测RANK mRNA表达量。结果 与本组第1天比较,两组第3天破骨细胞增殖均降低(P<0.01),TRAP酶活性增加(P<0.01),实验组第3天RANK mRNA表达降低(P<0.05);与本组第3天比较,第7天两组破骨细胞增殖能力增高(P<0.01)。与对照组比较,实验组第1天RANK mRNA表达更高(P<0.05),第3、7天破骨细胞增殖能力及RANK mRNA表达降低(P<0.05,P<0.01),第7天TRAP酶活性降低(P<0.01)。结论 大蒜素对破骨细胞分化成熟具有显著抑制作用,其对破骨细胞胞的增殖、RANK mRNA表达、破骨细胞活性作用均有抑制作用,且呈时间依赖性。Objective To study the effect of allicin regulating receptor activator of nuclear factor-κB(RANK) on osteoclast differentiation and maturation.Methods Mouse bone marrow mesenchymal stem cells(BMSCs)were induced to make osteoclast differentiation,then the cells were divided into control group and experimental group.At 1,3,7 days,the two groups were cultured by 30 ng/mL macrophage-stimulating factor(M-CSF)and 60 ng/mL RANKL,the experimental group was received allicin(20μg/m L) intervention.The proliferation of cells in the two groups was detected by CCK-8,the TRAP enzyme activity was detected by tartrate-resistant acid phosphatase(TRAP) staining and quantitative detection,and RANK mRNA expression was detected by RT-PCR.Results Compared with the 1st day,the proliferation of osteoclasts in the two groups decreased(P<0.01),the activity of TRAP enzyme increased(P<0.01),and the expression of RANK mRNA in the experimental group decreased on day 3(P<0.05).Compared with the 3rd day,the proliferation ability of osteoclasts in the two groups increased on the 7th day(P<0.01).Compared with the control group,RANK mRNA expression in the experimental group was higher on 1st day(P<0.05),osteoclast proliferation and RANK mRNA expression decreased on day 3 and 7(P<0.05,P<0.01),and TRAP enzyme activity decreased on 7th day(P<0.01).Conclusion Al icin has significant inhibitory effect on osteoclast differentiation and maturation,and has inhibitory effects on osteoclast cell proliferation,RANK mRNA expression and osteoclast activity in a time-dependent manner.

关 键 词：大蒜素 骨髓间充质干细胞 核因子ΚB受体活化因子 破骨分化 

分 类 号：R285[医药卫生—中药学]