油莎豆CeWRKY转录因子响应非生物胁迫的功能表征  

Functional characterization of Cyperus esculentus CeWRKY transcription factors in response to abiotic stress

作　　者：史先飞 高宇 黄旭升 周雅莉 蔡桂萍 李昕儒 李润植 薛金爱 SHI Xian-fei;GAO Yu;HUANG Xu-sheng;ZHOU Ya-li;CAI Gui-ping;LI Xin-ru;LI Run-zhi;XUE Jin-ai(College of Agronomy,Institute of Molecular Agriculture and Bioenergy,Shanxi Agricultural University,Jinzhong 030801,China)

机构地区：[1]山西农业大学农学院,分子农业与生物能源研究所,山西晋中030801

出　　处：《草业学报》2023年第8期186-201,共16页Acta Prataculturae Sinica

基　　金：国家自然科学基金(31401430,31902394,31201266);山西农业大学生物育种工程项目(YZGC101);山西省重点研发项目(201703D221002-3);山西省高校科技成果培育项目;山西省高校科技创新项目(2021L112);山西省基础研究项目(20210302124170);2021研究生创新项目(2021Y311)资助

摘　　要：油莎豆亦称油莎草,地上部分是优质牧草,地下块茎可积累大量油脂,抗逆性强、适应性广,是一种新型饲草和油料作物。WRKY是一类数量庞大的转录因子超家族,广泛参与调控植物生长发育、物质代谢和胁迫应答等生理过程。然而,有关于油莎豆WRKY家族成员及其功能知之甚少。本研究基于转录组分析,鉴定出67条油莎豆WRKY转录因子。系统发育树结果显示,油莎豆CeWRKY与拟南芥AtWRKY类似,分为I、Ⅱ、Ⅲ3个亚家族。CeWRKYs均具有典型的WRKY结构域和锌指结构域,其中7条蛋白序列的WRKY结构域存在变异,可能与WRKY成员功能歧化相关。油莎豆CeWRKY蛋白的序列长度、相对分子量、等电点等存在较大差异,均为亲水性蛋白,不含跨膜结构,亚细胞定位预测均位于细胞核。CeWRKY基因在块茎发育时期的表达模式具有时空特异性。不同CeWRKY基因参与不同逆境胁迫响应,其中CeWRKY8基因分别在干旱、盐、碱胁迫处理的油莎豆幼苗中表达谱变化显著,可能是参与油莎豆逆境胁迫响应调控的一个主效转录因子。本研究为深入解析油莎豆CeWRKY转录因子的多样性生物学功能以及介导逆境胁迫应答的分子机制奠定了基础,亦为优质高抗油莎豆新品种培育提供科学参考。Cyperus esculentus is a new type of forage and oil crop,with its overground leaves providing high-quality herbage and its underground tubers accumulating high levels of oleic acid-enriched oils.C.esculentus also possesses strong tolerance against various stresses.WRKY transcription factor(TF)is one group among the large superfamily of transcription factors involved in growth and development,tissue metabolism,stress response and other physiological processes in the plant.However,there is limited knowledge about the WRKY family members and their functions in C.esculentus.Here,a total of 67 CeWRKY transcription factors were identified by mining transcriptome data.Phylogenetic analysis showed that CeWRKYs were classified into three groups which we designated I,ⅡandⅢ.CeWRKYs have both the typical WRKY domain and a zinc finger domain.Seven CeWRKYs exhibited mutation in the WRKY domains,which may be related to their functional divergence.The sequence length,molecular weight,and isoelectric point of CeWRKY proteins are predicted to vary greatly.CeWRKY proteins are predicted to be hydrophilic proteins without a transmembrane structure.These proteins were predicted to be localized in the nucleus.Temporal and spatial expression of CeWRKY genes were detected during C.esculentus tuber development.Different CeWRKY genes displayed differential expression patters in C.esculentus seedlings in response to different stresses.The expression profile of the CeWRKY8 gene changed significantly in C.esculentus seedlings exposed to drought,salt,or alkali stresses.This CeWRKY8 may be a major regulator of stress response in C.esculentus.The present study establishes the essential framework for complete understanding of the diverse biological function of CeWRKY transcription factors and molecular mechanisms mediating stress response in C.esculentus,providing a scientific pathway for developing new varieties of C.esculentus with high quality,enhanced biomass yield and strong stress resistance.

关 键 词：油莎豆 WRKY转录因子 基因家族鉴定 表达模式 非生物胁迫 

分 类 号：Q943.2[生物学—植物学]