基于可见近红外光谱的牙周炎患者牙龈组织血氧含量分析  

Analysis of Blood Oxygen Content in Gingival Tissue of Patients With Periodontitis Based on Visible and Near-Infrared Spectroscopy

作　　者：郭园[1,2] 黄义翔[3,4] 黄长平 孙雪剑 栾庆先 张立福[3,5] GUO Yuan;HUANG Yi-xiang;HUANG Chang-ping;SUN Xue-jian;LUAN Qing-xian;ZHANG Li-fu(Department of Periodontology,Peking University School and Hospital of Stomatology&National Clinical Research Center for Oral Diseases&National Engineering Laboratory for Digital and Material Technology of Stomatology&Beijing Key Laboratory of Digital Stomatology,Beijing 100081,China;Second Dental Center,School and Hospital of Stomatology,Peking University,Beijing 100101,China;Aerospace Information Research Institute,Chinese Academy of Sciences,Beijing 100101,China;University of Chinese Academy of Sciences,Beijing 100049,China;Progoo Information Technology Co.,Ltd.,Tianjin 300392,China)

机构地区：[1]北京大学口腔医学院·口腔医院牙周科,国家口腔疾病临床医学研究中心,口腔数字化医疗技术和材料国家工程实验室,口腔数字医学北京市重点实验室,北京100081 [2]北京大学口腔医院第二门诊部,北京100101 [3]中国科学院空天信息创新研究院,北京100101 [4]中国科学院大学,北京100049 [5]天津中科谱光信息技术有限公司,天津300392

出　　处：《光谱学与光谱分析》2023年第8期2563-2567,共5页Spectroscopy and Spectral Analysis

基　　金：中国科学院青年创新促进会优秀会员(Y20211047);天津市智能制造专项资金项目(20211072);“科技助力经济2020”重点专项智能光谱检测系统的研发及示范应用项目资助。

摘　　要：牙周炎是牙齿周围软硬组织的一种感染性、破坏性疾病,主要临床表现为牙周袋的形成,临床附着丧失以及牙槽骨吸收。可见近红外光谱技术具有无创、快速检测等优势,在医学领域得到了较为广泛的应用。利用可见近红外光谱技术检测重度牙周炎牙龈组织内含氧血红蛋白和脱氧血红蛋白含量。本研究纳入5名健康对照者和5名重度牙周炎患者,采集健康者20个位点和重度牙周炎20个位点龈缘、龈缘下4 mm及龈缘下7 mm的牙龈组织光谱(400~1700 nm),研究发现含氧血红蛋白与脱氧血红蛋白分别在544和576 nm处表现出明显光谱吸收特征。对原始光谱数据进行包络线去除分析,并计算含氧血红蛋白和脱氧血红蛋白的相对吸收深度。结果表明重度牙周炎牙周袋内的含氧血红蛋白和脱氧血红蛋白含量均显著高于健康组(p<0.05),而重度牙周炎深牙周袋内不同深度位置其含氧血红蛋白和脱氧血红蛋白含量无统计学差异。研究结果可反映重度牙周炎与健康牙龈组织中血氧含量差异,为可见近红外光谱技术应用于牙周炎患者牙龈组织中含氧血红蛋白和脱氧血红蛋白含量的无创检测提供科学依据。Periodontitis is an infectious,destructive and inflammatory disease of periodontal tissue around teeth.The main clinical manifestations of periodontitis are soft tissue pocket formation,clinical attachment loss and alveolar bone resorption.Visible near-infrared spectroscopy,characterized by noninvasive and rapid detection,has been widely used in medicine.The present study explored the use of visible near-infrared spectroscopy in evaluating the relative contents of oxygenated hemoglobin and deoxyhemoglobin in gingival tissue of severe periodontitis.The gingival tissue spectra(400~1700 nm)were obtained and processed from 20 sites of 5 healthy subjects and 20 sites of 5 patients with severe periodontitis.Spectra were collected at the gingival margin,4 and 7 mm below the gingival margin.Our research found that oxygenated hemoglobin and deoxyhemoglobin showed obvious spectral absorption characteristics at 544 and 576 nm respectively.The relative absorption depths of oxygenated hemoglobin and deoxyhemoglobin were calculated from the continuum removal based on the original spectral data.The results showed that the relative contents of oxygenated hemoglobin and deoxyhemoglobin in the periodontal pocket of severe periodontitis were significantly higher than those in the healthy group(p<0.05).At the same time,there was no significant difference in the contents of oxygenated hemoglobin and deoxyhemoglobin at different depths in the deep periodontal pocket of severe periodontitis.The results reflected the hemodynamic differences between severe periodontitis and healthy gingival tissue,and provided a scientific basis for applying visible near-infrared spectroscopy in noninvasive detection and auxiliary diagnosis of periodontitis.

关 键 词：可见近红外光谱 无创检测 牙周炎 含氧血红蛋白 脱氧血红蛋白 

分 类 号：R78[医药卫生—口腔医学]