贝莱斯芽孢杆菌BzAH-1的抗菌特性及应用  

Antibacterial Properties and Application of Bacillus Velezensis BzAH-1

作　　者：尤巧 李焱辉 王彤 赵陆宇 彭文婷 王欣雨 林凌[1] 黄炜乾 YOU Qiao;LI Yanhui;WANG Tong;ZHAO Luyu;PENG Wenting;WANG Xinyu;LIN Ling;HUANG Weiqian(College of Life Sciences,Anhui Normal University,Anhui Wuhu 241000,China;Qingyuan Yisheng Natural Biology Research Institute Co.,Ltd.,Guangdong Qingyuan 511500,China)

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000 [2]清远一生自然生物研究院有限公司,广东清远511500

出　　处：《饲料工业》2023年第15期79-85,共7页Feed Industry

基　　金：安徽省高校自然科学研究项目[KJ2020A0066];清远市2022年省科技专项资金(“大专项+任务清单”)项目[2022DZX008]。

摘　　要：为了开发新型的抗生素替代品,试验分离鉴定了一株贝莱斯芽孢杆菌BzAH-1,对其产生的抗菌代谢产物的抑菌机制、耐性、应用效果等方面进行研究。通过发酵生产获得了其抗菌代谢物,测定其抑菌圈的大小。通过抑菌试验研究了抗菌代谢物的抑菌效果、热稳定性、酸碱耐受性以及可能的抑菌机制。结果表明:该抗菌代谢物质热稳定性较好,70℃水浴24 h对其活性无任何影响;对pH 2~11范围内的缓冲液耐受性较高;作为代谢物制剂添加到饲料中,能够显著提高蛋鸡产蛋率,降低料蛋比和死亡率。贝莱斯芽孢杆菌BzAH-1的代谢抗菌物具良好的抗菌活性、耐高温和耐酸碱性,具有较强的应用价值。In order to develop the new antibiotic substitutes,a strain of Bacillus velezensis BzAH-1 with antibacterial mechanism,tolerance,and application effects of its antibacterial metabolites were studied,and its antibacterial metabolite was obtained and determined by antibacterial zone.The bacteriostatic effect,thermal stability,acid-base tolerance and bacteriostatic mechanism were detected in this experiment.The results showed that the antibacterial metabolites had good thermal stability under the condition of 70℃for 24 h,while had high tolerance to acid-base buffers ranging in pH 2-11.As a probiotics,Bacillus velezensis BzAH-1 can improve the laying rate,feed conversion ratio and reduce mortality rate of laying hens.It indicated Bacillus velezensis BzAH-1 had good antibacterial activity,high temperature resistance,acid-alkali tolerance could be applied in aquaculture industry.

关 键 词：贝莱斯芽孢杆菌 益生菌 抑菌特性 蛋鸡 肠道 生产应用 

分 类 号：S816.73[农业科学—饲料科学]