Biomimetic melatonin-loaded silk fibroin/GelMA scaffold strengthens cartilage repair through retrieval of mitochondrial functions  

作　　者：Mingzhuang Hou Yijian Zhang Yang Liu Xiaoyang Ge Xiayu Hu Zhijian Zhao Xin Tian Tao Liu Huilin Yang Xi Chen Fan He Xuesong Zhu 

机构地区：[1]Department of Orthopaedics,The First Affiliated Hospital of Soochow University,Soochow University,Suzhou 215006,China [2]Orthopaedic Institute,Medical College,Soochow University,Suzhou 215007,China [3]Department of Pathology,The Third Affiliated Hospital of Soochow University,Changzhou 213003,China

出　　处：《Journal of Materials Science & Technology》2023年第15期102-112,共11页材料科学技术（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.82072410,82072476 and 82072442);the Natural Science Foundation of Jiangsu Province(No.BK20220046);the Major Science and Technology Project of Changzhou Health Commission(No.ZD202001);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).

摘　　要：Articular cartilage injury induced by collision or trauma is a common sports-related condition that may progress to pain,dysfunction,and secondary osteoarthritis(OA).Limited by self-renewal potential,cartilage regeneration faces a quandary while bio-inspired novel strategies are urgently required.In this study,by a soft freezing method and surface modification technique,a multi-functional silk fibroin(SF)plus gelatin methacrylate(GelMA)scaffold laden with melatonin(MT)was prepared.SF-GelMA@MT scaffold demonstrated enhanced biomechanical characteristics and long-acting melatonin release.In vitro treatment with SF-GelMA@MT induced the synthesis of cartilage extracellular matrix(ECM)components.Mechanistically,sustained release of melatonin yielded robust chondroprotective effects via improving mitochondrial polarization and antioxidant properties.SF-GelMA@MT implantation boosted cartilage renascence in a full-thickness cartilage defect model via mitochondria-associated sirtuins 1(SIRT1)-superoxide dismutase 2(SOD2)signaling pathway in vivo.In summary,this research proposed a welldesigned bionic composite scaffold that promotes cartilage regeneration via mitochondrial function enhancement,which is of tremendous potential for cartilage tissue engineering.

关 键 词：Cartilage injury Silk fibroin Gelatin methacrylate MELATONIN Mitochondri 

分 类 号：TQ127.11[化学工程—无机化工] TP212[自动化与计算机技术—检测技术与自动化装置]