几种提取方法制备的糙刺参体壁胶原蛋白的特性分析  

Characteristics of collagens from body wall of Stichopus horrens prepared by different extraction methods

作　　者：郑清瑶 曹文红 韩昱梁 张峰宁 陈忠琴 林海生 郑惠娜 ZHENG Qingyao;CAO Wenhong;HAN Yuliang;ZHANG Fengning;CHEN Zhongqin;LIN Haisheng;ZHENG Huina(College of Food Science and Technology,Guangdong Ocean University,National Research and Development Branch Center for Shellfish Processing(Zhanjiang),Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety,Guangdong Provincial Engineering Technology Research Center of Seafood,Guangdong Province Engineering Laboratory for Marine Biological Products,Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution,Zhanjiang 524088,China;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]广东海洋大学食品科技学院,国家贝类加工技术研发分中心(湛江),广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研究中心,广东省海洋生物制品工程实验室,水产品深加工广东普通高等学校重点实验室,广东湛江524088 [2]海洋食品精深加工关键技术省部共建协同创新中心,大连工业大学,辽宁大连116034

出　　处：《食品与发酵工业》2023年第15期145-152,共8页Food and Fermentation Industries

基　　金：国家重点研发计划课题项目(2020YFD0901104);湛江市农业技术攻关项目(2021A05187);广东省普通高校创新团队项目(2021KCXTD021)。

摘　　要：为考察不同提取方法对糙刺参体壁胶原蛋白分子结构和理化性质的影响,该研究以糙刺参体壁为研究对象,分别提取酸溶性胶原蛋白(acid-soluble collagen,ASC)、酶溶性胶原蛋白(pepsin-solubilized collagen,PSC)、超声波辅助酸溶性胶原蛋白(ultrasound-assisted extraction of acid-soluble collagen,UASC)和超声波辅助酶溶性胶原蛋白(ultrasound-assisted extraction of pepsin-solubilized collagen,UPSC),比较分析不同提取方法的提取率、SDS-PAGE、紫外光谱、红外光谱、热稳定性及圆二色光谱数据。结果显示,UPSC提取率最高,为18.70%,ASC提取率仅为0.15%,未用于后续研究;SDS-PAGE结果表明,PSC、UPSC、UASC的亚基组成均为(α1)2α2;红外光谱显示,3种胶原蛋白均存在酰胺A、B、Ⅰ、Ⅱ和Ⅲ带,均保持了胶原蛋白三螺旋结构的完整性,经超声波处理后,α螺旋含量降低,β折叠和β转角含量增加;PSC、UPSC、UASC的热变性温度分别为21.3、25.9、27.0℃;圆二色光谱和紫外光谱表明,3种胶原蛋白均符合Ⅰ型胶原蛋白的典型结构特征。综上,可初步判断糙刺参体壁胶原蛋白为Ⅰ型胶原蛋白,超声波辅助提取未破坏胶原蛋白的三螺旋结构,且能促进胶原蛋白的提取。研究结果可为糙刺参体壁胶原蛋白的后续开发利用提供依据。To investigate the effects of different extraction methods on the molecular structure and physicochemical properties of collagens from the body wall of Stichopus horrens,acid-soluble collagen(ASC),pepsin-solubilized collagen(PSC),ultrasound-assisted extraction of acid-soluble collagen(UASC),and ultrasound-assisted extraction of pepsin-solubilized collagen(UPSC)were extracted from the body wall of S.horrens,the extraction rate,SDS-PAGE,ultraviolet spectrum(UV),Fourier transform infrared spectrum(FTIR),thermal stability and circular dichroism spectrum(CD)of different extraction methods were compared and analyzed.Results showed that the extraction rate of UPSC was the highest(18.70%)and that of ASC was only 0.15%,which was not used in subsequent studies.SDS-PAGE showed that the subunit composition of PSC,UPSC,and UASC was(α1)2α2.FTIR spectrum showed that there were amide A,B,Ⅰ,Ⅱ,andⅢbands in all three kinds of collagens,which maintained the integrity of the triple helix structure of collagens,after ultrasonic treatment,the contents ofα-helix decreased,while the contents ofβ-pleated sheet andβ-turn increased.The thermal denaturation temperatures of PSC,UPSC,and UASC were 21.3℃,25.9℃,and 27.0℃,respectively.CD and UV spectrum showed that three kinds of collagens were consistent with the typical structural characteristics of typeⅠcollagen.In conclusion,it could preliminarily be judged that the collagens from the body wall of S.horrens were typeⅠcollagen,ultrasonic-assisted extraction did not destroy the triple helix structure of collagens,it could promote the extraction of collagens.This study could provide basic data for the further development and utilization of collagens from the body wall of S.horrens.

关 键 词：糙刺参 胶原蛋白 提取方法 结构特征 理化性质 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]