对羟基苯甲酸-壳聚糖接枝物的合成及性能研究  

Synthesis and properties of p -hydroxybenzoic acid-chitosan conjugate

作　　者：王皎龙 WANG Jiaolong(College of Food Science and Engineering,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学食品科学与工程学院,海南海口570228

出　　处：《食品与发酵工业》2023年第15期187-192,共6页Food and Fermentation Industries

基　　金：海南省科技专项资助项目(ZDYF2022XDNY156);海南省高等学校科学研究项目(Hnky2022-5)。

摘　　要：采用自由基接枝方法合成了不同接枝度的对羟基苯甲酸-壳聚糖(p-hydroxybenzoic acid-chitosan,PA-g-CS),以改善其应用性能。通过紫外-可见光谱、红外光谱、X射线衍射、热重分析和扫描电子显微镜对PA-g-CS进行表征,并考察其体外抗氧化和抑菌性能。结果表明,PA-g-CS分子中对羟基苯甲酸(p-hydroxybenzoic acid,PA)与壳聚糖(chitosan,CS)的羟基和氨基共价相连。与CS相比,PA-g-CS的结晶度和热稳定性降低,表面形态变为疏松多孔。体外抗氧化和抑菌实验表明,PA-g-CS的DPPH自由基清除活性,对革兰氏阴性(大肠杆菌)和阳性细菌(金黄色葡萄球菌)的抑制能力均显著增强。高接枝度的PA-g-CS抗氧化和抑菌活性更为优异。PA-g-CS有望应用于抗氧化剂和防腐剂等领域。In this study,p-hydroxybenzoic acid-grafted chitosan(PA-g-CS)conjugates with different grafting degrees were synthesized by free radical-regulated grafting approach,so as to improve its application performance.The obtained PA-g-CS were characterized by UV-Vis spectra,FT-IR spectra,X-rays diffraction,thermogravimetric analysis and scanning electron microscopy.Results revealed that PA was successfully grafted onto the hydroxyl group and the amino group positions of CS.Compared with CS,PA-g-CS showed decreased crystallinity,thermal stability,as well as a loose and porous surface morphology.The antioxidant assays demonstrated that PA-g-CS exhibited remarkably enhanced DPPH-scavenging activities in vitro.Similarly,the synthesized PA-g-CS also significantly increased the antimicrobial activities toward Gram negative and positive bacteria,such as Escherichia coli and Staphylococcus aureus.Moreover,the products with a relatively higher grafting degree showed stronger biological activities.Our findings suggest that PA-g-CS conjugates have great potential application in the field of active packaging,antioxidants,and preservatives.

关 键 词：壳聚糖 对羟基苯甲酸 结构表征 生物活性 

分 类 号：TS202.3[轻工技术与工程—食品科学]