共价有机框架材料的合成及其在酶固定化中的应用进展  

Progress on the synthesis of covalent organic framework materials and their application in enzyme immobilization

作　　者：张衡 杨帆[1] 任瑞鹏[1] 吕永康[1] Zhang Heng;Yang Fan;Ren Ruipeng;Lv Yongkang(State Key Laboratory of Clean and Efficient Coal Utilization,Taiyuan University of Technology,Taiyuan 030024)

机构地区：[1]太原理工大学,省部共建煤基能源清洁高效利用国家重点实验室,太原030024

出　　处：《化工新型材料》2023年第7期37-42,共6页New Chemical Materials

基　　金：国家自然科学基金(21776196和51778397);山西省应用基础研究计划(20210302124431)。

摘　　要：酶是一类具备优异催化活性和高区域选择性的天然催化剂,在工业催化领域具有广阔的应用前景。然而,酶促转化的应用受到酶在恶劣条件下的脆弱性的阻碍。幸运的是,固定化已被证明是改善酶不稳定性一种有前途的方法。由于物理化学特性可调,高结晶度,大比表面积、优越的吸附能力、可预先设计的结构和出色的稳定性等特性,共价有机框架(COFs)被认为是固定化酶的理想载体材料。与其他传统载体材料相比,COFs的高度多孔性使其具有更高的酶载量,表现出更优的催化效果。酶是通过物理吸附、直接共价连接或通过交联剂,实现与COFs的结合。首先介绍了COFs的合成方法与功能化,然后系统总结了COFs固定化酶的策略与最新研究进展,最后初步讨论了COFs在固定化酶领域面临的挑战与机遇。Enzymes are natural catalysts with remarkable catalytic activity and high regioselectivity and have promising applications in industrial catalysis.However,the application of enzymatic conversion is hindered by the vulnerability of enzymes under harsh conditions.Fortunately,immobilization has been shown to be a promising approach to improve enzyme instability.Covalent organic frameworks(COFs)are considered as ideal carrier materials for immobilizing enzymes due to their tunable physicochemical properties,high crystallinity,large specific surface area,superior adsorption capacity,pre-designable structure and excellent stability.Compared with other conventional carrier materials,the highly porous nature of COFs allows them to have higher enzyme loading capacity and exhibit superior catalytic effects.The binding of enzymes to COFs is achieved by physical adsorption,direct covalent linkage,or by cross-linking agents.In this paper,we firstly introduced the synthesis methods and functionalization of COFs,then systematically summarized the strategies and recent research progress in COFs immobilizing enzymes,and finally,we initially discussed the challenges and opportunities of COFs in the field of enzyme immobilization.

关 键 词：共价有机框架 固定化酶 物理吸附 共价相互作用 生物催化剂 

分 类 号：TB34[一般工业技术—材料科学与工程] Q814.2[生物学—生物工程]