Enzymatic Synthesis of Sialyl Lactosamine Grafted Chitooligosaccharides  

作　　者：Jun Yan Jianghua Li Zhifei Hu Xiaoyao Ma Yun Li Xihuan Hu Jinfeng Ye Wei Wang Renzhong Wan Hongzhi Cao 

机构地区：[1]College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Taian,Shandong,271018 China [2]Laboratory for Marine Drugs and Bioproducts,National Laboratory for Marine Science and Technology (Qingdao),Qingdao,Shandong,266237 China [3]Key Laboratory of Marine Drugs of Ministry of Education,Shandong Key Laboratory of Glycoscience and Glycotechnology,School of Medicine and Pharmacy,Ocean University of China,Qingdao,Shandong,266003 China

出　　处：《Chinese Journal of Chemistry》2023年第11期1299-1304,共6页中国化学（英文版）

基　　金：supported by the National Key Research and Development Program of China(2021YFC210500 and 2022YFC2104900);the National Natural Science Foundation of China(21961142016,22277111 and 22107094);Department of Science and Technology of Shandong Province(2020CXGC010601,2021ZDSYS22 and ZR2021QB061)。

摘　　要：An efficient enzymatic assembly strategy was developed for the concise synthesis of structurally well-defined sialylated chitooligosaccharides.Two enzyme modules for theβ-1,3-N-acetyl-glucosaminylation andβ-1,4-galactosylation were applied for the grafting N-acetyl lactosamine(LacNAc)unit(s)onto the chitooligosaccharides.The LacNAc grafted chitooligosaccharides were further modified withα-2,3-orα-2,6-sialylation by two enzymatic sialylation modules to generate a total of 20 sialylated chitooligosaccharides.The inhibition study of influenza virus-induced cytopathy with synthetic sialylated chitooligosaccharides indicated that the sialic acid linkage and chain length both contribute to the binding preference and inhibition potency.

关 键 词：CHITOOLIGOSACCHARIDES Sialylation Influenza virus GLYCOSYLATION Biocatalysis Structure-activity relationships 

分 类 号：O62[理学—有机化学]