牦牛DRA基因克隆测序及生物信息学分析  

Cloning sequencing and bioinformatics analysis of DRA gene of yak

作　　者：张丽[1] 王媛媛 王瑞 刘丽霞[1] ZHANG Li;WANG Yuanyuan;WANG Rui;LIU Lixia(College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730030,China)

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730030

出　　处：《浙江农业学报》2023年第7期1564-1570,共7页Acta Agriculturae Zhejiangensis

基　　金：西北民族大学中央高校基本科研业务费资金资助项目(31920200074,31920210061);西北民族大学研究生科研创新项目(Yxm2021074)。

摘　　要：为全面解析牦牛MHCⅡ类基因结构与功能,采用分子克隆测序技术对大通牦牛DRA基因编码区进行等位基因频率估算并进行生物信息学分析。结果显示:大通牦牛DRA基因编码区含有762 bp的开放阅读框,共编码253个氨基酸;共有3个SNPs(g.2376C>T、g.2851C>G、g.3016C>A),其中第2、3外显子分别存在1个C→T的同义突变和C→A的错义突变(L→M),这两个突变均降低了大通牦牛DRA基因mRNA二级结构的稳定性;g.3016C>A突变前后编码蛋白质的分子量、原子总数、脂肪系数、不稳定系数、总平均亲水性方面存在差异;生物信息学的方法分析显示,BoLA-DRA基因是MHC基因家族中高度保守的基因。该研究结果可以为大通牦牛DRA基因结构与功能的研究奠定基础。In order to comprehensively analyze the structure and function of yak MHCⅡgene,molecular cloning and sequencing technology was used to estimate the coding region of DRA gene of Datong yak and conduct bioinformatics analysis.The results showed a DRA gene open reading frame in the coding region containing 762 bp,a total of 253 amino acids encoding;a total of 3 SNPs(g.2376C>T,g.2851C>G,g.3016C>A).In exons 2 and 3,there was one synonymous mutation in C→T and a missense mutation(L→M)in C→A,respectively,both mutations reduced the stability of mRNA secondary structure of DRA gene.There were differences in the molecular mass,total number of atoms,fat coefficient,instability coefficient,and total average hydrophilicity of the protein encoded by the g.3016C>A mutation.Methodological analysis by bioinformatics revealed that the BoLA-DRA gene were highly conserved genes in the MHC gene family.The results laid the foundation for the structure and function of DRA in Datong yak.

关 键 词：DRA基因 编码区克隆 突变位点 生物信息学 大通牦牛 

分 类 号：S823.85[农业科学—畜牧学]