磁性固定化辣根过氧化物酶脱除玉米赤霉烯酮的研究  

Studies on the Removal of Zearalenone from Maize Using Magnetic ImmobilizedHorseradish Peroxidase

作　　者：屈岩峰 于殿宇[2] Qu Yanfeng;Yu Dianyu(Harbin University,Harbin 150086;College of Food Science,Northeast Agricultural University,Harbin 150030;Jiusan Oils and Grains Industries Group Co.,Ltd.,Harbin 150090;High-skilled Talents(Model Worker)Innovation Studio of Qu Yanfeng,Harbin 150086)

机构地区：[1]哈尔滨学院,哈尔滨150086 [2]东北农业大学食品学院,哈尔滨150030 [3]九三粮油工业集团有限公司,哈尔滨150090 [4]屈岩峰高技能人才(劳模)创新工作室,哈尔滨150086

出　　处：《中国食品学报》2023年第7期229-239,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：黑龙江省“百千万”工程科技重大专项(2020ZX 08B01);哈尔滨市科技计划自筹经费项目(2022ZCZJCG033)。

摘　　要：本文制备了磁性Fe_(3)O_(4)-SiO_(2)-HPG-COOH复合载体作为固定化载体,共价固定辣根过氧化物酶(HRP),应用于玉米赤霉烯酮(ZEN)的降解。利用傅里叶红外光谱、X-射线衍射分析、扫描电镜等表征方法对固定化HRP进行表征,验证了HRP被成功固定在载体上。制备的纳米级载体Fe_(3)O_(4)-SiO_(2)-HPG-COOH具有均匀的尺寸和形状,良好的分散性和较强的磁性,饱和磁化值为25.80 emu/g,平均粒径为103.15 nm。与游离酶相比,固定化HRP具有更高的耐酸碱性和热稳定性,最适pH值从6.5提高到7.0,最适温度从30℃提升到35℃。将磁性固定化HRP应用于ZEN降解,利用单因素实验,探究反应温度、过氧化氢浓度、溶液pH值和反应时间与ZEN降解率的关系。结果表明:在反应温度35℃,过氧化氢浓度26 mmol/L,溶液pH 7.0,反应时间480 min条件下,ZEN的脱除效率为68%,固定化HRP具有良好的重复使用性,循环5次后相对酶活力仍保持在79.85%以上。研究表明磁性固定化HRP对ZEN具有良好的降解能力。In this paper,magnetic nanoparticles Fe_(3)O_(4)-SiO_(2)-HPG-COOH was prepared as immobilized carrier to covalently immobilize horseradish peroxidase(HRP),and applied to the degradation of zearalenone(ZEN).The immobilized HRP was characterized by Fourier transform infrared spectroscopy,X-ray diffraction and scanning electron microscopy,which verified that HRP was successfully immobilized on the carrier.The prepared nanoscale carrier Fe_(3)O_(4)-SiO_(2)-HPG-COOH has uniform size and shape,good dispersion and strong magnetic properties.The saturation magnetization value was 25.80 emu/g,and the average particle size was 103.15 nm.Compared with free enzyme,the magnetically immobilized HRP indicated higher acid-base tolerance and thermal stability,the optimal pH value was increased from 6.5 to 7.0,the optimal temperature was increased from 30℃to 35℃.Magnetic immobilized HRP was applied to ZEN degradation,and the relationship between solution pH,reaction temperature,hydrogen peroxide concentration and reaction time with ZEN degradation rate was investigated using single-factor experiments.The results showed that under the conditions of reaction temperature of 35℃,hydrogen peroxide concentration of solution 26 mmol/L,pH 7.0 and reaction time 480 min,the removal efficiency of ZEN was 68%.The immobilized HRP had good repeatability,and the relative enzyme activity remained above 79.85%after 5 cycles.This research shows that magnetic immobilized HRP has good degradation ability to ZEN.

关 键 词：辣根过氧化物酶 磁性固定化 玉米赤霉烯酮 脱除效率 重复使用 

分 类 号：TS210.4[轻工技术与工程—粮食、油脂及植物蛋白工程]