青稞麦麸γ-氨基丁酸的制备条件优化  

Optimization of γ-Aminobutyric Acid Preparing Conditions Using Highland Barley Bran

作　　者：江迪 张紫晋[1] 杨婷 赵爽[1,4] 彭君 蒲邓佳 陈静 Jiang Di;Zhang Zijin;Yang Ting;Zhao Shuang;Peng Jun;Pu Dengjia;Chen Jing(Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041;Sichuan Agricultural University,Ya'an 625014;Sichuan 3Institute of Resources and Environment,Tibet Academy of Agriculture and Animal Husbandry Sciences,Lhasa 850000;Yunnan Agricultural University,Kunming 650500)

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]四川农业大学,四川雅安625014 [3]西藏自治区农牧科学院农业资源与环境研究所,拉萨850000 [4]云南农业大学,昆明650500

出　　处：《中国食品学报》2023年第7期259-266,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：四川省区域创新合作项目(2020YFQ0001);中国科学院科技服务网络计划项目(KFJ-STS-QYZD-117);西藏科技重点研发项目(XZDZKJ-2021-01-06,XZ202201ZD0001N)。

摘　　要：以青稞麸皮为原料,利用内源谷氨酸脱羧酶(GAD)和外源添加谷氨酸钠及磷酸吡哆醛(PLP)富集γ-氨基丁酸(GABA)。在比较不同品种GABA富集能力差异的基础上,采用以pH值、料液比、外源谷氨酸钠和磷酸吡哆醛浓度、反应温度和时间为变量的单因素实验和正交试验方法,得到GABA富集的优化工艺条件。结合青稞麸皮分级制备GABA的比较试验结果,建立GABA的高效制备方法:麸皮脱皮率4.23%~6.43%,料液比140∶1(g/L),反应时间12 h,反应温度35℃,添加谷氨酸钠浓度为8 mmol/L,PLP浓度为18μmol/L。利用该方法获得的GABA最高产量为19.57 mmol/L,外源底物转化率在97%以上。A method forγ-aminobutyric acid(GABA)production was studied using highland barley bran combined with adding sodium glutamate and pyridoxal 5'-phosphate(PLP).Based on the influences of different varieties on GABA bioconversion and the single test of factors including solution pH,initial whole flour concentration,reaction temperature and time,initial concentrations of sodium glutamate and PLP,the optimal reaction conditions of GABA production through the orthogonal experiment were developed.This research further showed that a strong influence of peeling level of barley bran on GABA enrichment.A safe and highly efficient preparing conditions without any buffer was determined as follows:bran peeling rate 4.23%-6.43%,barley bran concentration 140∶1(g/L),reaction time 12 h,reaction temperature 35℃,8 mmol/L and 18μmol/L supplemental level of sodium glutamate and PLP,respectively.The optimal reactions produced 19.57 mmol/L GABA from 8 mmol/L sodium glutamate,with a GABA conversion rate up to 97%.

关 键 词：青稞麦麸 Γ-氨基丁酸 正交试验 工艺条件 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]