Anti-inflammatory and anabolic biphasic scaffold facilitates osteochondral tissue regeneration in osteoarthritic joints  

作　　者：Xiangbo Meng Ling Li Cuishan Huang Keda Shi Qingqiang Zeng Chunyi Wen Sibylle Grad Mauro Alini Ling Qin Xinluan Wang 

机构地区：[1]Translational Medicine R&D Center,Institute of Biomedical and Health Engineering,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China [2]AO Research Institute Davos,Clavadelerstrasse 8,Davos Platz 7270,Switzerland [3]Musculoskeletal Research Laboratory,Department of Orthopaedics & Traumatology,The Chinese University of Hong Kong,Hong Kong SAR 999077,China [4]CAS-HK Joint Lab of Biomaterials,Joint Laboratory of Chinese Academic of Science and Hong Kong for Biomaterials,Translational Medicine Research and Development Center,Shenzhen Institutes of Advanced Technology of Chinese Academy of Sciences and The Chinese University of Hong Kong,China [5]Department of Biomedical Engineering,Faculty of Engineering,The Hong Kong Polytechnic University,Hong Kong SAR 999077,China

出　　处：《Journal of Materials Science & Technology》2023年第25期20-31,共12页材料科学技术（英文版）

基　　金：supported by the collaborative project from the National Key R&D Program of China and Innovation and Tech-nology Fund Mainland-Hong Kong Joint Funding Scheme(Nos.2021YFE0202300 and MHP/011/20);the Sino-Swiss collaborative project from the Ministry of Science and Technology and the Swiss National Science Foundation under the SSSTC program(Grant Nos.2015DFG32200 and 156362);Shenzhen Collaborative Innovation Plan-International Cooperation Project(Grant No.GJHZ20190821160803823);Development and Reform Commission of Shenzhen Municipality(2019)(No.561);Shenzhen Double Chain Project for Innovation and Development Industry supported by Bureau of Industry and Information Technology of Shenzhen(No.201908141541).

摘　　要：Osteochondral defects (OCD) are common but difficult to heal due to the low intrinsic repair capacity of cartilage and its complex hierarchical structure. In osteoarthritis (OA), OCD become more challenging to repair as both cartilage and subchondral bone regeneration are further impaired due to the arthritic environment. Numerous biomaterials have been developed and tested in osteochondral defects while ignoring the inflammatory environment. To target this challenging underlying pathophysiology, we designed and fabricated a biphasic porous and degradable scaffold incorporating anti-inflammatory and anabolic molecules by low-temperature rapid prototyping technology, and its effects on promoting osteochondral regeneration were evaluated using our well-established OA-OCD rabbit model. The biphasic porous scaffolds consisted of poly lactic-co-glycolic acid (PLGA) with kartogenin (KGN) for cartilage repair and PLGA and β-calcium phosphate (PLGA/β-TCP) with cinnamaldehyde (CIN) for subchondral bone repair. KGN is a molecule for promoting chondrogenesis and CIN is a phytomolecule for enhancing osteogenesis and alleviating inflammation. The biphasic scaffolds PLGA/KGN-PLGA/β-TCP/CIN (PK/PTC) with bio-mimic structure provided stable mechanical properties and exhibited excellent biocompatibility to support cell adhesion, proliferation, migration, and distribution. Furthermore, KGN and CIN within biphasic scaffolds could be released in a controlled and sustained mode, and the biphasic scaffold degraded slowly in vitro . Evaluating the repair of 16-weeks post-implantation into critically sized OA-OCD rabbit models revealed that the biphasic scaffold could promote subchondral bone and cartilage regeneration, as well as reverse subchondral osteosclerosis caused by inflammation in vivo . These findings support the utilization of the PK/PTC scaffold for osteochondral regeneration and provide a promising potential strategy for clinical application for the treatment of patients with OA-OCD.

关 键 词：Biphasic scaffold Osteochondral defect Osteoarthritis Kartogenin CINNAMALDEHYDE ANTI-INFLAMMATION 

分 类 号：TB383.1[一般工业技术—材料科学与工程] R318.08[医药卫生—生物医学工程]