海洋放线菌Nocardiopsis sp.HM-01的抑菌活性及抑菌物质鉴定  

Antimicrobial Activity and Substances of Nocardiopsis sp.HM-01

作　　者：许慧敏 成颖 崔萌菲 王依婷 李居行 肖逸飞 李贞景[1] 郭庆彬 刘欢欢 XU Huimin;CHENG Ying;CUI Mengfei;WANG Yiting;LI Juxing;XIAO Yifei;LI Zhenjing;GUO Qingbin;LIU Huanhuan(School of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学食品科学与工程学院,天津300457

出　　处：《食品研究与开发》2023年第16期40-47,共8页Food Research and Development

基　　金：国家自然科学基金青年科学基金项目(31800072)。

摘　　要：该研究针对一株海洋放线菌HM-01进行形态学及16S rDNA分析,并对该菌株的发酵液提取物进行抑菌特性研究,采用antiSMASH对该菌株亲缘性较高的基因组序列进行次级代谢产物基因簇预测,并结合液相色谱-质谱分析,鉴定主要抑菌活性物质。结果表明,该菌株被鉴定为拟诺卡氏菌属(Nocardiopsis)。Nocardiopsis sp.HM-01发酵液提取物对大肠杆菌、金黄色葡萄球菌、鳗弧菌、阴沟肠杆菌、铜绿假单胞菌等均有明显抑制活性,且以RP培养基在发酵第9天时效果最明显。鉴定出主要的抑菌活性物质为desferrioxamine E和icosalide B。The morphological characteristics and 16S rDNA sequence of a marine actinomycete strain HM-01 and the antibacterial properties of the fermentation extract were studied.Furthermore,antiSMASH was employed to predict the gene clusters for the synthesis of secondary metabolites based on the homologous genome sequences.The main antibacterial substances were identified by liquid chromatography-mass spectrometry.The strain was identified as Nocardiopsis.The fermentation extract of Nocardiopsis sp.HM-01 exhibited significantly inhibitory activities against Escherichia coli,Staphylococcus aureus,Vibrio anguillarum,Enterobacter cloacae,and Pseudomonas aeruginosa,with the highest activity on day 9 of fermentation with RP medium.The main antibacterial substances were identified as desferrioxamine E and icosalide B.

关 键 词：拟诺卡氏菌属 海洋放线菌 抑菌谱 液相色谱-质谱 次级代谢产物 基因组挖掘 

分 类 号：TS201.3[轻工技术与工程—食品科学]