natural polymer-based bilayer adhesive skin substitute for infected burned wound healing  

作　　者：Mina Shahriari-Khalaji Mamoona Sattar Ran Cao Meifang Zhu 

机构地区：[1]State Key Laboratory for Modification of Chemical Fibers and Polymer Materials,College of Materials Science and Engineering,Donghua University,Shanghai,201620,China [2]Research Group of Microbiological Engineering and Medical Materials,College of Biological Science and Medical Engineering,Donghua University,Shanghai,201620,China [3]Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine,Donghua University,Shanghai,201620,China

出　　处：《Bioactive Materials》2023年第11期177-195,共19页生物活性材料（英文）

基　　金：the National Key Research and Development Program of China(2021YFA1201304/2021YFA1201300);the Fundamental Research Funds for the Central Universities(2232022D-01);the Science and Technology Commission of Shanghai Municipality,China(20DZ2254900);the Young Elite Scientists Sponsorship Program by CAST(YESS20220259).

摘　　要：Thermal wounds are complex and lethal with irregular shapes, risk of infection, slow healing, and large surface area. The mortality rate in patients with infected burns is twice that of non-infected burns. Developing multifunctional skin substitutes to augment the healing rate of infected burns is vital. Herein, we 3D printed a hydrogel scaffold comprising carboxymethyl chitosan (CMCs) and oxidized alginate grafted catechol (O-AlgCat) on a hydrophobic electrospun layer, forming a bilayer skin substitute (BSS). The functional layer (FL) was fabricated by physiochemical crosslinking to ensure favorable biodegradability. The gallium-containing hydrophobic electrospun layer or backing layer (BL) could mimic the epidermis of skin, avoiding fluid penetration and offering antibacterial activity. 3D printed FL contains catechol, gallium, and biologically active platelet rich fibrin (PRF) to adhere to both tissue and BL, show antibacterial activity, encourage angiogenesis, cell growth, and migration. The fabricated bioactive BSS exhibited noticeable adhesive properties (P ≤ 0.05), significant antibacterial activity (P ≤ 0.05), faster clot formation, and the potential to promote proliferation (P ≤ 0.05) and migration (P ≤ 0.05) of L929 cells. Furthermore, the angiogenesis was significantly higher (P ≤ 0.05) when evaluated in vivo and in ovo. The BSS-covered wounds healed faster due to low inflammation and high collagen density. Based on the obtained results, the fabricated bioactive BSS could be an effective treatment for infected burn wounds.

关 键 词：Infected burn wound Bioactive skin substitute Double cross-linked ANTI-INFLAMMATION Tissue adhesion 

分 类 号：R318.08[医药卫生—生物医学工程]