基于高通量测序的不同填料生物滤池细菌群落结构分析  

Analysis of bacterial community structure in biofilters with different fillers using high-throughput sequencing

作　　者：陈小红[1] 郑惠东[1] 仇登高[1] 林永青[1] 许贻斌[1] 陈财珍[1] CHEN Xiaohong;ZHENG Huidong;QIU Denggao;LIN Yongqing;XU Yibin;CHEN Caizhen(Fujian Fishery Research Institute,Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province,National and Local Joint Engineering Research Center of Marine Biological Seed Industry Technology,Xiamen 361013,China)

机构地区：[1]福建省水产研究所,福建省海洋生物增养殖与高值化利用重点实验室,海洋生物种业技术国家地方联合工程研究中心,福建厦门361013

出　　处：《海洋环境科学》2023年第4期550-557,共8页Marine Environmental Science

基　　金：福建省海洋与渔业结构调整专项(2021HYJG12、2021HYJG08);福建省海洋经济发展专项资金项目(FJHJF-L-2021-9)。

摘　　要：为探究不同填料的挂膜特性,分别构建4组填料生物滤池(珊瑚石、悬浮球填料、弹性填料、组合填料),比较其自然挂膜成熟时间,并利用Illumina MiSeq高通量测序技术分析其细菌群落结构。结果表明,珊瑚石、悬浮球填料、弹性填料、组合填料的挂膜成熟时间分别为15 d、18 d、21 d、21 d。不同生物滤池中填料生物膜上的细菌丰度和多样性均高于对应的生物滤池水体,悬浮球填料生物滤池的多样性指数最高。在门分类水平上,不同生物滤池的优势菌群主要以变形菌门(Proteobacteria)为主。在属分类水平上,不同生物滤池的脱氮功能菌有所不同,亚硝酸菌属(Nitrosomonas)、硝化刺菌属(Nitrospina)在弹性填料生物滤池中的相对丰度大于其他生物滤池;芽孢杆菌属(Bacillus)是珊瑚石生物滤池的优势菌属;亚硫酸杆菌属(Sulfitobacter)、栖海杆菌属(Maribacter)、Phaeodactylibacter是悬浮球填料生物滤池特有的具有反硝化作用的优势菌属。总体上,不同填料生物滤池均有较完整的硝化-反硝化脱氮微生物体系,这些微生物在海水养殖尾水脱氮处理中具有重要作用。In order to investigate the biofilm characteristics of different fillers,four different fillers,including coral stone,suspended ball filler,elastic filler and combined filler,were used to build biofilters.The mature time of biofilms was compared,and the bacterial community structure of filler-biofilms and seawater in biofilters were analyzed using Illumina Miseq high-throughput sequencing technology.Our results show that the mature time of biofilms using coral stone,suspended ball,elastic,or combined filler were 15 d,18 d,21 d and 21 d,respectively.The bacterial abundance and diversity on biofilms of the filler were higher than the seawater of their corresponding biofilter.The diversity index of the suspended ball biofilter was the highest among different biofilters.At the taxonomic level,Proteobacteria was the dominant bacteria in all biofilters.At the genus classification level,different biofliters showed different characteristics of denitrifying functional bacteria.The relative abundance of Nitrosomonas and Nitrospina in elastic filler biofilter was the highest among different biofilters.Bacillus was the dominant genus of coral stone biofilter.The dominant denitrifying bacteria of sulfitobacter,maribacter and phaeodactylibacter were the characteristic bacteria of the suspended ball biofilter.In general,biofilters using different fillers showed a relatively complete nitrification and denitrification microbial system,which played an important role in the denitrification treatment of mariculture wastewater.

关 键 词：海水养殖尾水 生物滤池 填料 细菌群落结构 

分 类 号：X172[环境科学与工程—环境科学]