谱系示踪探索肝实质细胞再生修复机制的研究进展  

Research progress in lineage tracing to explore hepatic parenchymal cell regeneration and repair mechanisms

作　　者：李丽[1] 王萍[1] Li Li;Wang Ping(Liver Disease Center,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院肝病中心,北京100050

出　　处：《中华肝脏病杂志》2023年第7期781-784,共4页Chinese Journal of Hepatology

基　　金：国家自然科学基金(81570548)。

摘　　要：肝实质细胞是肝脏承担代谢、解毒等重要功能的细胞类型,由于其具有很强的增殖能力使得肝脏损伤修复过程中肝实质细胞、胆管细胞和肝脏干/祖细胞对新生肝实质细胞的贡献程度成为了有争议的问题。谱系示踪技术是近十余年兴起的、探索细胞起源的新方法,通过对表达某一基因的细胞及其后代细胞进行标记可以实现对特定类型细胞及其子代细胞进行示踪。现对利用该技术探索肝实质细胞起源与贡献的文献进行综述,在肝脏稳态和急性损伤后,约98%的新生肝实质细胞来源于原有肝实质细胞,在肝实质细胞增殖被抑制等严重肝损伤的情况下,胆管细胞(主要是其中的肝脏干/祖细胞)才成为肝实质细胞的主要来源,且其贡献率随时间延长逐渐增加。Hepatic parenchymal cells are a type of liver cells that performs important functions such as metabolism and detoxification.The contribution of hepatic parenchymal cells,bile duct cells,and hepatic stem/progenitor cells to new hepatic parenchymal cells in the process of liver injury repair has become a controversial issue due to their strong proliferation ability.Lineage tracing technology,which has emerged in the past decade as a new method for exploring the origin of cells,can trace specific type of cells and their daughter cells by labeling cells that express the specific gene and their progeny.The article reviews the current literature on the origin and contribution of hepatic parenchymal cells by this technique.About 98%of new hepatic parenchymal cells originate from the existing hepatic parenchymal cells during liver homeostasis and after acute injury.However,under conditions of severe liver injury,such as inhibition of hepatic parenchymal cell proliferation,bile duct cells(mainly liver stem/progenitor cells)become the predominant source of hepatic parenchymal cells,contributing a steady increased hepatocyte regeneration with the extension of time.

关 键 词：肝细胞 肝实质细胞再生 谱系示踪 胆管细胞 肝脏干/祖细胞 

分 类 号：R575[医药卫生—消化系统]