In vivo antioxidant activity of rabbiteye blueberry(Vaccinium ashei cv.'Brightwell')anthocyanin extracts  

作　　者：Jing WANG Xingyu ZHAO Jiawei ZHENG Daniela D.HERRERA-BALANDRANO Xiaoxiao ZHANG Wuyang HUANG Zhongquan SUI 

机构地区：[1]College of Chemical Engineering,Nanjing Forestry University,Nanjing 210037,China [2]Institute of Agro-Product Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China [3]School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China [4]School of Life Sciences,Nantong University,Nantong 226007,China [5]Department of Food Science&Engineering,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2023年第7期602-616,共15页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the Jiangsu Provincial Key Research and Development Program(No.BE2021705),China。

摘　　要：Blueberries are rich in phenolic compounds including anthocyanins which are closely related to biological health functions.The purpose of this study was to investigate the antioxidant activity of blueberry anthocyanins extracted from'Brightwell'rabbiteye blueberries in mice.After one week of adaptation,C57BL/6J healthy male mice were divided into different groups that were administered with 100,400,or 800 mg/kg blueberry anthocyanin extract(BAE),and sacrificed at different time points(0.1,0.5,1,2,4,8,or 12 h).The plasma,eyeball,intestine,liver,and adipose tissues were collected to compare their antioxidant activity,including total antioxidant capacity(T-AOC),superoxide dismutase(SOD)activity and glutathione-peroxidase(GSH-PX/GPX)content,and the oxidative stress marker malondialdehyde(MDA)level.The results showed that blueberry anthocyanins had positive concentration-dependent antioxidant activity in vivo.The greater the concentration of BAE,the higher the T-AOC value,but the lower the MDA level.The enzyme activity of SOD,the content of GSH-PX,and messenger RNA(mRNA)levels of Cu,Zn-SOD,Mn-SOD,and GPX all confirmed that BAE played an antioxidant role after digestion in mice by improving their antioxidant defense.The in vivo antioxidant activity of BAE indicated that blueberry anthocyanins could be developed into functional foods or nutraceuticals with the aim of preventing or treating oxidative stress-related diseases.

关 键 词：Blueberry anthocyanin In vivo antioxidant activity Superoxide dismutase(SOD) Glutathione-peroxidase(GSH-PX/GPX) Malondialdehyde(MDA) 

分 类 号：TS201.2[轻工技术与工程—食品科学]