RIL群体中大豆籽粒脂肪酸组分QTL分析  

QTL Analysis of Fatty Acid Composition in Soybean Seeds from RIL Population

作　　者：王茜 刘兵强[2] 贾馨元 杨庆 黄冀楠 史晓蕾[2] 杨春燕[2] 陈庆山[1] WANG Xi;LIU Bingqiang;JIA Xinyuan;YANG Qing;HUANG Jinan;SHI Xiaolei;YANG Chunyan;CHEN Qingshan(College of Agriculture,Northeast Agricultural University,Harbin 150030,China;Institute of Cereal and Oil Crops,Hebei Academy of Agricultural and Forestry Sciences/Shijiazhuang Branch Center of National Center for Soybean Improvement/Huang-Huai-Hai Key Laboratory of Biology and Genetic Improvement of Soybean,Ministry of Agriculture and Rural Affairs/The Key Laboratory of Crop Genetics and Breeding,Shijiazhuang 050035,China;College of Life Science,Hengshui College,Hengshui 053000,China)

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]河北省农林科学院粮油作物研究所/国家大豆改良中心石家庄分中心/农业农村部黄淮海大豆生物学与遗传育种重点实验室/河北省作物遗传育种实验室,河北石家庄050035 [3]衡水学院生命科学学院,河北衡水053000

出　　处：《大豆科学》2023年第4期416-423,共8页Soybean Science

基　　金：国家大豆产业技术体系(CARS04);河北省农林科学院科技创新专项课题(2022KJCXZX-LYS-7);河北省农林科学院院博士后基金(C22R0309);河北省现代农业产业技术体系(HBCT2019190201);大豆现代种业科技创新团队(21326313D);河北省高端人才专项。

摘　　要：大豆是世界上主要的油料作物,其脂肪酸各组分的比例决定了大豆油的质量。本研究以“徐豆16×冀HJ117”构建的包含183个家系的重组自交系(Recombinant Inbred Lines,RILs)群体XH1617为材料,通过气相色谱法检测棕榈酸、硬脂酸、油酸、亚油酸、亚麻酸5种脂肪酸含量。表型变异分析显示,RIL群体的5种脂肪酸含量均呈近似正态分布,说明大豆5种脂肪酸含量均为典型的数量性状。通过QTL定位,共鉴定到13个与大豆脂肪酸含量相关的QTL位点,分别位于05、06、08、13、14、15和19号染色体上,LOD值为2.50~12.35,可解释的表型变异范围为6.1%~26.7%。其中6个QTL是新鉴定到的与大豆脂肪酸含量相关的遗传位点。另外,在15号染色体上鉴定到1个主效且协同调控大豆棕榈酸含量和油酸含量的遗传位点,Locus_15_POA,在标记Gm15_3702534_C_T~Gm15_4491175_T_C之间,LOD值为4.71~5.11,可解释的表型变异为11.20%~12.10%。相关性分析发现,除硬脂酸含量与油酸含量无显著性相关外,其他脂肪酸含量之间均具有显著相关性(P<0.05)。研究结果可为培育高油酸且低棕榈酸的高品质大豆提供遗传材料和理论依据。As a major oil crop in the word,the proportion of each fatty acids determined the quality of seed oil in soybean.In this study,XH1617,a set of recombinant inbred lines(RILs)population consisting of 183 lines and were derived from a cross between Xudou 16 and HJ117,were used as the experimental materials.The contents of five fatty acids,palmitic,stearic,oleic,linoleic and linolenic acids,were determined by gas chromatograph.The phenotypic variation analysis showed that these five fatty acids contents approximately showed a normal distribution,suggesting that the contents of five faty acids were typical quantitative traits.The results of QTL mapping showed that a total of 13 QTLs associated with soybean fatty acids were identified on chromosome 05,06,08,13,14,15 and 19,respectively.And the L0D were ranged from 2.50 to 12.35,explaining 6.1%-26.7%of phenotypic variation.Among them,6 new QTLs associated with soybean fatty acids were identified.In addition,one main-effect QTL,Locus_15_POA,synergistically regulating the content of palmitic acid and oleic acid,was identified between the makers of Gm15_3702534_C_T and Gm15_4491175_T_C on chromosome 15.Its L0D were ranged from 4.71 to 5.11,explaining 11.20%-12.10%of phenotypic variation.Pearson correlation analysis showed that there were significantly differences among the fatty acids,except for the stearic acid content and oleic acid content(P<0.05).In conclusion,these results could provide theoretical basis and genetic resources for breeders to develop new soybean varieties with high oleic acid content and low palmitic acid content.

关 键 词：大豆 脂肪酸含量 QTL定位分析 遗传分析 相关性分析 

分 类 号：S565.1[农业科学—作物学]