紫苏bZIP转录因子全基因组鉴定及对非生物胁迫的响应分析  

Genome-wide characterization and abiotic stress response analysis of bZIP transcription factors in Perilla frutescens

作　　者：黄旭升 周雅莉[1] 史先飞 高宇 董书言 蔡桂萍 李润植[1] 王计平[1] HUANG Xusheng;ZHOU Yali;SHI Xianfei;GAO Yu;DONG Shuyan;CAI Guiping;LI Runzhi;WANG Jiping(College of Agronomy,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China)

机构地区：[1]山西农业大学农学院,山西晋中030801

出　　处：《植物生理学报》2023年第7期1383-1397,共15页Plant Physiology Journal

基　　金：国家自然科学基金(31201266和31401430);山西省重点研发计划项目(201603D312005和201803D221005-9);山西省晋中市科技重点研发计划项目(Y172007-5);山西省基础研究计划(自由探索类)项目(20210302123418);山西农业大学农学院育种工程专项重点培育项目(YZ2021-08)。

摘　　要：碱性亮氨酸拉链(bZIP)是一类普遍存在于真核生物的转录因子,广泛参与植物生长发育与逆境胁迫响应等生命过程。为解析紫苏(Perilla frutescens)bZIP(PfbZIP)蛋白的生物学功能,本研究应用组学工具全基因组鉴定PfbZIP家族成员及其理化特征,定量PCR检测PfbZIP基因响应非生物胁迫的表达谱,克隆PfbZIP90编码序列,并进行烟草(Nicotiana tabacum)遗传转化。结果表明,紫苏基因组共鉴定到101个PfbZIP家族成员,不均匀分布于18条染色体。PfbZIP蛋白序列长度在112~1269 aa之间,等电点(pI)为4.84~10.88。主要由二级结构单元α-螺旋和无规则卷曲维持PfbZIP蛋白自身结构。亚细胞定位预测PfbZIP定位于细胞核。片段重复是PfbZIP基因家族进化和扩增的主要驱动力。不同PfbZIP成员之间外显子和内含子数目及分布差异较大。PfbZIP蛋白具有高度保守的碱性区域和亮氨酸拉链结构区域。系统进化分析将PfbZIP家族成员划分为10个亚群。PfbZIP基因启动子区包含大量非生物胁迫响应的顺式元件。表达谱分析显示部分PfbZIP基因表达水平在低温和干旱胁迫的紫苏幼苗以及对照植株中差异显著,其中PfbZIP90在低温和干旱胁迫下均显著上调。与非转基因对照相比,异源超表达PfbZIP90的转基因烟草株系可溶性糖含量升高,电导率和丙二醛含量则显著降低,Nt FAD3基因表达和α-亚麻酸(ALA)含量亦显著增加。这表明PfbZIP90能有效介导紫苏和烟草低温和干旱胁迫应答以及脂肪酸合成调控。这些发现为全面探究PfbZIP家族成员的生物学功能,特别是介导非生物胁迫响应的分子调控机制和油脂品质改良提供了新的科学基础。The basic leucine zipper(bziP),a class of transcription factors ubiquitous in eukaryotes,is widely involved in various life processes such as plant growth,development and stress responses.To reveal the biological functions of PfbziP proteins in Perlla frutescens,omics tools were employed to identify Pfb-ZiP gene family members and their physicochemical properties.Quantitative PcR was used to examine expression profiles of PfbZiP genes in response to abiotic stress.The coding sequence of PfbZiP90 was cloned,and then was genetically transformed into tobacco(Nicotiana tabacum).The results showed that total of 101 PfbziP family members were identified from the P.frutescens genome,with uneven distributions on 18 chromosomes.The amino acid numbers of PfbZiP proteins ranged from 112 to 1269 aa,and the isoelectric point(pl)was between 4.84 and 10.88.a-helix and random coil were detected as the two main secondary structure units to maintain PfbziP protein integraty.Subcellular localization prediction indicated that PfbziPs were located in the nucleus.Segment duplication is detected to be the key evolutionary driving force of PfbziP gene family expansion and functional divergence.The distribution and number of exons and introns varied widely among different PfbZIP members.All PfbZiP proteins had highly-conserved basic regions and leucine zipper structure regions.PfbZiP family members were clustered into 10 subgroups by phylogenetic analysis.The promoter analysis indicated that PfbziP genes contained multiple abiotic stress response cis-elements.A number of PfbziP genes were revealed to have different expression levels in low temperature and drought stressed perilla seedlings compared to the control plants.Pfb-ZiP90 was significantly up-regulated in perilla seedlings under both low temperature and drought stresses.Transgenic tobacco lines heterologously overexpressing PfbziP90 exhibited an increase in soluble sugar content but a significant reduction in electrical conductivity and malondialdehyde content compared to the non

关 键 词：紫苏 碱性亮氨酸拉链(bZIP) PfbZIP基因家族 非生物胁迫 烟草遗传转化 Α-亚麻酸 

分 类 号：S565.9[农业科学—作物学]