微塑料与双酚A联合下鲤鱼生物积累及氧化应激效应  

Interactive effects of polystyrene microplastics and bisphenol A on bioaccumulation and oxidative stress in the Yellow River carp(Cyprinus carpio haematopterus)

作　　者：侯晨丽 于越 于玲红[1,2] 王志超 于晟乾 李卫平 HOU Chen-li;YU Yue;YU Ling-hong;WANG Zhi-chao;YU Sheng-qian;LI Wei-ping(School of Energy and Environment,Inner Mongolia University of Science and Technology,Baotou 014010,China;Collaborative Innovation Center of Autonomous Region for Ecological Protection and Comprehensive Utilization in the Inner Mongolia Section of the Yellow River Basin,Baotou 014010,China)

机构地区：[1]内蒙古科技大学能源与环境学院,内蒙古包头014010 [2]黄河流域内蒙古段生态保护与综合利用自治区协同创新中心,内蒙古包头014010

出　　处：《中国环境科学》2023年第8期4334-4342,共9页China Environmental Science

基　　金：内蒙古自治区自然科学基金资助项目(2020MS02017);黄河流域内蒙古段复杂河湖环境治理技术应用与示范(2021GG0410)。

摘　　要：为了阐明微塑料(MPs)对双酚A(BPA)在鱼体内分布和生物积累的影响,以及两者之间交互的毒理效应,选用黄河鲤鱼为模型生物,分析0.5μm聚苯乙烯微塑料(PS-MPs)与BPA联合下鲤鱼不同组织中PS-MPs和BPA的富集特征、组织病理及抗氧化酶活性.结果表明:暴露21d后,鲤鱼各组织中PS-MPs的富集量均为肠道(55.70%)>鳃(31.53%)>大脑(7.76%)>肝脏(5.01%).与BPA处理相比,BPA+MP20处理中鳃、肝脏、肠道和大脑中的BPA浓度分别增加4.28%、1.66%、2.06%和4.85%;BPA+MP100处理分别增加11.88%、15.18%、3.15%和10.54%,表明PS-MPs的存在显著增加了BPA在鲤鱼各组织中的积累,且各组织中BPA积累量随PS-MPs浓度的增加而增加,其中肝脏中BPA积累量对PS-MPs浓度依赖性更强.相较于对照处理,单一BPA或MPs与BPA联合作用均引起鲤鱼各组织出现不同程度炎症细胞浸润,并诱导各组织中的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽(GSH)活性及丙二醛(MAD)含量增加.然而,BPA与高浓度PS-MPs联合作用下,鲤鱼通过诱导增加肠道和肝脏中SOD和GSH活性,降低MAD含量,减缓MPs和BPA对鲤鱼的复合毒性效应.The purpose of the present study was to elucidate the effects of MPs(microporous)on the distribution and bioaccumulation of BPA(bisphenol A)in fish as well as their interactive toxicological effects in fish.Yellow River carp(Cyprinus carpio haematopterus)was employed as the model organism.In this study,the tissues accumulation characteristics of PS-MPs and BPA,histopathology and antioxidant enzyme activities of Yellow River carp under the combined with(0.5μm)PS-MPs and BPA were analyzed.The enrichment of PS-MPs followed the order of gut(55.70%)>gill(31.53%)>brain(7.76%)>liver(5.01%)in various tissues of carp after 21days of exposure.Compared with BPA treatment,BPA concentrations of BPA+MP20treatment in gill,liver,gut and brain were increased by 4.28%,1.66%,2.06% and 4.85%,respectively,increased by 11.88%,15.18%,3.15% and 10.54% in BPA+MP100 treatments,respectively.The presence of PS-MPs significantly increased the accumulation of BPA in carp tissues,and the accumulation of BPA in tissues increased with the increase of the concentration of PS-MPs,and the accumulation of BPA in liver was more dependent on the concentration of PS-MPs.Compared with the control treatment,single BPA or MPs combined with BPA could cause different degrees of inflammatory cell infiltration in each tissue of carp,and induce the increase of superoxide dismutase(SOD),catalase(CAT),glutathione(GSH)activity and malondialdehyde(MAD)content in each tissue.However,interactive effects of BPA and high concentration of PS-MPs,the SOD and GSH activities in the intestine and liver of carp were induced to decrease the content of MAD,to mitigate the combined toxic effects of microplastics and bisphenol A on carp.

关 键 词：微塑料 双酚A 联合暴露 累积量 抗氧化酶活性 

分 类 号：X171.5[环境科学与工程—环境科学]