石油污染土壤对蚯蚓氧化应激响应研究  

Oxidative stress responseresponse of petroleum-contaminated soil to earthworm

作　　者：欧阳少虎 黄盼盼 陶宗鑫 周启星[1] OUYANG Shao-hu;HUANG Pan-pan;TAO Zong-xin;ZHOU Qi-xing(Key Laboratory of Pollution Processes and Environmental Criteria(Ministry of Education),College of Environmental Science and Engineering,Nankai University,Tianjin 300071,China;Environmental Protection Agency,Dongying 257000,China)

机构地区：[1]南开大学环境科学与工程学院,环境污染过程与基准教育部重点实验室,天津300071 [2]东营市生态环境局,山东257000

出　　处：《中国环境科学》2023年第8期4353-4359,共7页China Environmental Science

基　　金：NSFC-山东联合基金重点项目(U1906222);国家重点研发计划项目(2019YFC1804104);国家自然科学基金青年基金(42107306);博士后面上项目(2020M680867)。

摘　　要：以黄河三角洲胜利油田典型石油污染土壤为研究对象,赤子爱胜蚓(Eisenia foetida)为受试生物,采用28d毒性直接暴露试验,对石油烃污染土壤的生态毒性进行研究,为土壤生态毒理诊断提供支撑.结果表明,蚯蚓暴露于不同浓度(0~10g/kg)石油烃污染土壤7,14,21和28d之后,蚯蚓体内抗氧化酶活性和丙二醛(MDA)含量对于暴露时间响应不明显.而对于暴露浓度的响应,蚯蚓体内超氧化物歧化酶(SOD),过氧化氢酶(CAT),过氧化物酶(POD)的活性变化趋势没有统一的规律,不能一概而论.而当不同浓度石油烃污染土壤胁迫时,蚯蚓体内MDA的含量与石油烃的暴露浓度呈良好的线性正相关(R2>0.85).因此,蚯蚓MDA含量适于石油烃污染土壤胁迫下的生物标志物用以指示脂质过氧化水平反应.The ecological toxicity of petroleum-contaminated soil of the Yellow River Delta on the earthworm(Eisenia foetida,test organisms)were investigated under 28d toxicity exposure,and then the ecotoxicity threshold of petroleum hydrocarbon in soil environment was preliminarily deduced,which could provide support for soil ecotoxicology diagnosis.The results showed that the activities of antioxidant enzymes and content of MDA in Eisenia foetida had no obvious response to exposure time after exposure to different concentrations(0~10g/kg)petroleum hydrocarbon contaminated soil for 7d,14d,21d and 28d.In response to the exposure concentration,the activities of SOD,CAT and POD in earthworms didn't have a uniform trend and couldn't be generalized.However,there was a positive linear correlation between the content of MDA in earthworms and the exposure concentration of petroleum hydrocarbons(R2>0.85).Therefore,MDA content of earthworm could be used as a biomarker to indicate lipid peroxidation under petroleum hydrocarbon contaminated soil stress.

关 键 词：石油污染 土壤 蚯蚓 生物指示物 生态毒性 

分 类 号：X53[环境科学与工程—环境工程]