NOD-Scid小鼠播散性弥漫大B细胞淋巴瘤模型的构建  

Establishment of a disseminated diffuse large B-cell lymphoma model in NOD-Scid mice

作　　者：闻照凤 黄绮堂 胡泉泉 李雨 李延莉 WEN Zhao-feng;HUANG Qi-tang;HU Quan-quan;LI Yu;LI Yan-li(Department of Pathology,School of Basic Medical Sciences,Anhui Medical University,Hefei 230032,China;Department of Pathology,the Second Affiliated Hospital of Zhejiang University School of Medicine,Hangzhou 310009,China;Department of Pathology,the Affiliated Anqing Hospital of Anhui Medical University/Anqing Municipal Hospital,Anqing 246003,China;The Second Clinical Medical College of Anhui Medical University,Hefei 230032,China;Department of Pathology,the First Affiliated Hospital of Anhui Medical University,Hefei 230022,China)

机构地区：[1]安徽医科大学基础医学院病理学教研室,合肥230032 [2]浙江大学医学院附属第二医院病理科,杭州310009 [3]安徽医科大学附属安庆医院(安庆市立医院)病理科,安庆246003 [4]安徽医科大学第二临床医学院,合肥230032 [5]安徽医科大学第一附属医院病理科,合肥230022

出　　处：《临床与实验病理学杂志》2023年第7期793-796,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(81700194);安徽省高校自然科学基金重点项目(KJ2021A0214);安徽省大学生创新创业训练计划项目(S202210366049)。

摘　　要：目的利用免疫缺陷的NOD-Scid小鼠建立播散性弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)小鼠模型,为动物模型的构建提供新思路。方法先将人源DLBCL细胞OCI-Ly8导入荧光素酶基因,使其长期稳定表达荧光素酶。再将OCI-Ly8-luc^(+)细胞经尾静脉注射到NOD-Scid小鼠体内,利用生物发光成像系统每隔3天观察1次小鼠体内肿瘤细胞播散情况。结果实验第7天监测到OCI-Ly8-luc^(+)细胞在小鼠体内发生播散,并且进展迅速,20天时出现小鼠死亡。结论利用OCI-Ly8-luc^(+)细胞尾静脉注射成功构建了NOD-Scid小鼠DLBCL模型,为DLBCL的动物模型构建提供新方法。Purpose To establish a mouse model of disseminated diffuse large B-cell lymphoma using combined immunodeficient NOD-Scid mice to provide new ideas for the construction of animal models.Methods Firstly,luciferin gene was introduced into human-derived diffuse large B-cell lymphoma cells OCI-Ly8 cells to make them express luciferin stably for a long time.Then OCI-Ly8-luc^(+)cells were injected into NOD-Scid mice via tail vein,and the tumor cell dissemination in mice was observed every 3 days using bioluminescence imaging system.Results Dispersal of OCI-Ly8-luc^(+)cells in mice began to be monitored on day 7 and progressed rapidly,with death occurring on day 20.Conclusion The NOD-Scid mouse model of diffuse large B-cell lymphoma is successfully established by using OCI-Ly8-luc^(+)cells via tail vein injection,which provides a new method for the construction of animal models in the study of diffuse large B-cell lymphoma.

关 键 词：淋巴瘤 弥漫大B细胞淋巴瘤 荧光素酶 NOD-Scid小鼠 动物模型 生物发光成像 

分 类 号：R733.4[医药卫生—肿瘤]