VEGFR2、miR-21在非小细胞肺癌中的表达及其与预后的关系  

Expression of VEGFR2 and miR-21 in non-small cell lung cancer and relationship with prognosis

作　　者：朱金红 王丽丽[2] 王希波 ZHU Jin-hong;WANG Li-li;WANG Xi-bo(People's Hospital of Zhucheng,Zhucheng SHANDONG 262200,China;Affiliated Hospital of Qingdao University,Qingdao SHANDONG 266000,China)

机构地区：[1]诸城市人民医院,山东诸城262200 [2]青岛大学附属医院,山东青岛266000

出　　处：《中国新药与临床杂志》2023年第7期467-473,共7页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的 探讨VEGFR2、mi R-21在非小细胞肺癌(NSCLC)中的表达及其与患者预后的关系。方法 回顾性分析本院行肺切除术的52例NSCLC患者临床资料。采用免疫组化法和实时PCR法测定肺癌组织及癌旁组织VEGFR2、mi R-21的表达情况,并比较表达差异。建立受试者工作特征(ROC)曲线分析mi R-21在NSCLC中的诊断效能,分析VEGFR2、mi R-21表达与患者病理特征的关系。采用K-M法分析VEGFR2、mi R-21表达与患者预后的关系。结果 NSCLC患者癌组织VEGFR2高表达率(46%vs. 13%)及mi R-21相对表达量水平(1.03±0.36 vs.0.62±0.25)明显高于癌旁组(P <0.05)。mi R-21诊断NSCLC的ROC曲线下面积为0.833(95%CI:0.755~0.910),cut-off值为0.870,灵敏度为61.5%,特异度为90.4%。不同年龄、性别、病理类型、肿瘤最大径、分化程度、T分期、N分期、淋巴结转移情况患者VEGFR2及mi R-21表达情况比较均无显著差异(P> 0.05),有无远处转移患者VEGFR2表达比较无显著差异(P> 0.05),有远处转移患者mi R-21高表达率高于无远处转移患者(P <0.05)。VEGFR2、mi R-21高表达患者生存期较低表达患者均明显缩短(P <0.05)。结论 VEGFR2、mi R-21在NSCLC患者癌组织中均有高表达,VEGFR2、mi R-21高表达会缩短患者生存时间,影响患者预后。AIM To investigate the expression of VEGFR2 and miR-21 in non-small cell lung cancer(NSCLC)and their correlation with prognosis.METHODS The clinical data of 52 NSCLC patients who underwent pneumonectomy in our hospital were retrospectively analyzed.The expressions of VEGFR2 and miR-21 in lung cancer tissues and adjacent tissues were detected by immunohistochemistry and real-time PCR,and the expression differences were compared.The receiver operating characteristic(ROC)curve was established to analyze the diagnostic efficacy of miR-21 in NSCLC,and the relationship between the expression of VEGFR2 and miR-21 and the pathological characteristics of patients was analyzed.The relationship between the expression of VECFR2 and miR-21 and the prognosis of patients were analyzed by K-M method.RESULTS The high expression rate of VECFR2(46%vs.13%)and the relative expression level of miR-21(1.03±0.36 vs.0.62±0.25)in cancer tissues of NSCLC patients were significantly higher than those in adjacent tissues(P<0.05).The area under the curve of miR-21 in the diagnosis of NSCLC was 0.833(95%CI:0.755-0.910),the cut-off value was 0.870,the corresponding sensitivity was 61.5%,and the specificity was 90.4%.There was no significant difference in the expression of VEGFR2 and miR-21 in patients with different age,gender,pathological type,tumor maximum diameter,degree of differentiation,T stage,N stage and lymph node metastasis(P>0.05).There was no significant difference in the expression of VEGFR2 in patients with or without distant metastasis(P>0.05).The high expression rate of miR-21 in patients with distant metastasis was significantly higher than that in patients without distant metastasis(P<0.05).The survival time of patients with high expression of VECFR2 and miR-21 was significantly shorter than that of patients with low expression(P<0.05).CONCLUSION VEGFR2 and miR-21 are highly expressed in cancer tissues of NSCLC patients.The high expression of VEGFR2 and miR-21 can shorten the survival time of patients and affect the prognosi

关 键 词：癌 非小细胞肺 血管内皮生长因子类 预后 MIR-21 

分 类 号：R737.2[医药卫生—肿瘤]