自循环稀土纳米荧光探针用于生物样本中抗坏血酸检测  

Self-Recyclable Rare-Earth Fluorescent Nanoprobe for Ascorbic Acid Detection in Biosamples

作　　者：刘瑜鑫 李萝园 魏征 周晶 Liu Yuxin;Li Luoyuan;Wei Zheng;Zhou Jing(Department of Chemistry,Capital Normal University,Beijing 100048,China)

机构地区：[1]首都师范大学化学系,北京100048

出　　处：《中国稀土学报》2023年第4期802-809,I0005,共9页Journal of the Chinese Society of Rare Earths

基　　金：北京市属高校高水平教师队伍建设支持计划-青年拔尖个人项目(CIT&TCD201904082)资助。

摘　　要：通过化学键连法在氨基化SiO_(2)包覆的NaLuF_(4):Yb,Er@NaLuF_(4)核壳结构纳米探针表面修饰水杨酸-铁配合物制备得到自循环稀土纳米荧光探针。观察到探针在540和1530 nm两处荧光发射峰的比值随抗坏血酸浓度增高而增大,并在0~1.0 mmol·L^(-1)浓度范围内呈现良好的线性关系。将与抗坏血酸反应后的探针静置一定时间后离心分离,可实现探针的循环利用,并在10次循环内保证荧光信号比值恢复至与抗坏血酸反应前的水平(比值变化<5%)。通过构建小鼠模型,并使用这一探针测定其血清和尿液中抗坏血酸浓度,初步验证了评估生物样本中抗坏血酸水平的可行性。Self-recyclable rare-earth fluorescent nanoprobes were prepared by chemically bonding salicylic acid-Fe^(Ⅲ) complex on the surface of NH_(2)-functionalized SiO_(2) coated NaLuF_(4):Yb,Er@NaLuF_(4) core-shell nanophosphors.It is observed that the ratio of the fluorescence intensity between 540 and 1530 nm increases as concentration of ascorbic acid increases,showing good linear relationship in the range of 0-1.0 mmol·L^(-1).The used nanoprobe can be recycled by letting it stand still and then centrifugating,possessing outstanding recyclable properties in 10 cycles.Further experiments confirmed the possibility of using this nanoprobe to evaluate ascorbic acid level in biosamples by determining ascorbic acid concentration in serum and urine of established mice model.

关 键 词：稀土 纳米探针 可循环 生物检测 抗坏血酸 

分 类 号：O614.33[理学—无机化学]