Nesfatin-1通过PI3K/Akt信号通路促进结直肠癌进展研究  

Nesfatin-1 promotes the progression of colorectal cancer through the PI3K/Akt pathway

作　　者：梁慧婷 东靓 郗彦凤 白文启 张振君 马晓博 周晶晶 刘艳虹 孙泽雨 雷立健 胡晓琴 LIANG Hui-ting;DONG Liang;XI Yan-feng;BAI Wen-qi;ZHANG Zhen-jun;MA Xiao-bo;ZHOU Jing-jing;LIU Yan-hong;SUN Ze-yu;LEI Li-jian;HU Xiao-qin(School of Public Health,Shanxi Medical University,Taiyuan,Shanxi 030001,China;不详)

机构地区：[1]山西医科大学公共卫生学院流行病学教研室,山西太原030001 [2]山西医科大学附属肿瘤医院 [3]山西医科大学第一附属医院

出　　处：《现代预防医学》2023年第14期2656-2662,共7页Modern Preventive Medicine

基　　金：国家自然科学基金(81803326);山西省应用基础研究项目(201801D221265);山西省高等学校教学改革创新项目(J20220372);山西省研究生教育改革研究课题(2020YJJG134,2018JG47);山西省华晋骨科公益基金会(20211120,20221116);中华医学会医学教育分会和中国高等教育学会医学教育专业委员会医学教育研究课题(2020A-N02036)。

摘　　要：目的探讨Nesfatin-1与结直肠癌(CRC)发生发展的相关性及潜在分子机制。方法RT-qPCR和WB法检测CRC患者癌及癌旁组织中Nesfatin-1的水平。采用CCK8、Transwell小室及流式细胞术检测SW480细胞敲低或过表达Nesfatin-1前后的增殖、侵袭、迁移及凋亡能力,WB法检测PI3K/Akt信号通路相关蛋白的表达。结果与癌旁组织相比,CRC癌组织中Nesfatin-1 mRNA和蛋白表达均上调(P=0.046,P=0.028)。Nesfatin-1在组织中的高表达与患者肿瘤分化程度(P=0.045)和淋巴结转移(P=0.045)相关。Nesfatin-1的沉默抑制SW480细胞的增殖、侵袭和迁移,促进凋亡,并下调p-PI3K和p-Akt的表达(P<0.001,P<0.001),而Nesfatin-1过表达结果则相反(P=0.016,P<0.001)。结论Nesfatin-1在CRC中表达上调并与肿瘤分化程度和淋巴结转移相关,促进CRC细胞增殖、侵袭和迁移,减少细胞凋亡,该作用可能与其激活PI3K/Akt通路相关。Objective To investigate the correlation between Nesfatin-1 and the occurrence and development of colorectal cancer and its potential molecular mechanism.Methods RT-qPCR and Western Blot(WB)were used to detect Nesfatin-1 levels in colorectal cancer and adjacent tissues.The cell proliferation,invasion,migration and apoptosis ability of SW480 cells before and after knockdown or overexpression of Nesfatin-1 were detected by CCK8,Transwell chamber and flow cytometry methods.The expression of PI3K/Akt signaling pathway-related proteins was detected by WB.Results Compared with the adjacent tissues,the mRNA and protein expression of Nesfatin-1 were up-regulated in human colorectal cancer tissues(P=0.046,P=0.028).High expression of Nesfatin-1 in tissues was associated with tumor differentiation(P=0.045)and lymph node metastasis(P=0.045).Knockdown of Nesfatin-1 could inhibit the proliferation,invasion and migration of SW480 cells,promote apoptosis,and down-regulated the expression of p-PI3K and p-Akt(P<0.001,P<0.001).The result of Nesfatin-1 overexpression was the opposite(P=0.016,P<0.001).Conclusion Nesfatin-1 expression is up-regulated in colorectal cancer and is associated with tumor differentiation and lymph node metastasis.It promotes the proliferation,invasion and migration of colorectal cancer cells and reduces cell apoptosis,which may be related to the activation of the PI3K/Akt pathway.

关 键 词：NESFATIN-1 结直肠癌 PI3K/AKT 

分 类 号：R735.34[医药卫生—肿瘤]