人参皂苷Rh2对大鼠C6胶质瘤细胞Siah-1、Synaptophysin、MMP9及VEGF表达的影响  

Impacts of Ginsenoside Rh2 on the Siah-1,Synaptophysin,MMP9 and VEGF Expression in Rat C6 Glioma Cells

作　　者：吴国玉[1] 杨俊[1] 熊绍权[1] 祝捷 李建梅 WU Guo-yu;YANG Jun;XIONG Shao-quan;ZHU Jie;LI Jian-mei(First Department of Oncology,Affiliated Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan,610075,China)

机构地区：[1]成都中医药大学附属医院肿瘤一科,四川成都610075

出　　处：《现代生物医学进展》2023年第13期2419-2423,共5页Progress in Modern Biomedicine

基　　金：四川省中医药管理局科学技术研究专项课题(2021MS445)。

摘　　要：目的:探讨人参皂苷Rh2对大鼠C6胶质瘤细胞Siah-1、突触素(Synaptophysin)、基质金属蛋白酶-9(MMP-9)血管内皮生长因子(VEGF)表达的影响。方法:将大鼠C6胶质瘤细胞分为对照组、人参皂苷Rh2低剂量组(16μg/m L)、人参皂苷Rh2中剂量组(32μg/m L)、人参皂苷Rh2高剂量组(48μg/m L),CCK-8法和平板克隆实验检测细胞增殖;流式细胞术检测细胞凋亡;Transwell检测细胞侵袭;实时荧光定量-聚合酶链式反应(qRT-PCR)检测C6胶质瘤细胞中VEGF、Siah-1、Synaptophysin、MMP-9 m RNA表达;蛋白质印迹法(Western blot)检测C6胶质瘤细胞中VEGF、Siah-1、Synaptophysin、MMP-9蛋白表达。结果:与对照组比较,人参皂苷Rh2低剂量组、人参皂苷Rh2中剂量组、人参皂苷Rh2高剂量组大鼠C6胶质瘤细胞OD_(450)值(24 h、48 h)、克隆形成率、细胞侵袭数、VEGF、Synaptophysin、MMP-9 m RNA及蛋白表达降低,细胞凋亡率、Siah-1 m RNA及蛋白表达升高,且呈剂量依赖性(P<0.05)。结论:人参皂苷Rh2可能通过上调Siah-1,下调VEGF、Synaptophysin、MMP-9表达来抑制大鼠C6胶质瘤细胞增殖与侵袭,促进细胞凋亡。Objective:To investigate the impacts of ginsenoside Rh2 on the Siah-1,synaptophysin,matrix metalloproteinase-9(MMP-9)and vascular endothelial growth factor(VEGF)expression in rat C6 glioma cells.Methods:Rat C6 glioma cells were grouped into control group,low-dose ginsenoside Rh2 group(16μg/mL),medium dose ginsenoside Rh2 group(32μg/mL),and high-dose ginsenoside Rh2 group(48μg/mL),cell proliferation was detected by CCK-8 method and plate cloning test.Cell apoptosis was detected by flow cytometry.Cell invasion was detected by Transwell.The VEGF,Siah-1,Synaptophysin and MMP-9 mRNA expression in C6 glioma cells was detected by real-time fluorescence quantitation-polymerase chain reaction(qRT-PCR);and Western blot was used to detect the protein VEGF,Siah-1,Synaptophysin and MMP-9 expression in C6 glioma cells.Results:Compared with the control group,the OD_(450)value(24 h,48 h),clone formation rate,cell invasion number,VEGF,Synaptophysin,MMP-9 mRNA and protein expression in C6 glioma cells in the low-dose ginsenoside Rh2 group,the medium dose ginsenoside Rh2 group,and the high-dose ginsenoside Rh2 group were decreased,the cell apoptosis rate and the Siah-1 mRNA and protein expression were increased,which were dose dependent(P<0.05).Conclusion:Ginsenoside Rh2 may inhibit the proliferation and invasion of rat C6 glioma cells and promote cell apoptosis by up-regulating Siah-1 and down-regulating the VEGF,Synaptophysin and MMP-9 expression.

关 键 词：人参皂苷RH2 胶质瘤 Siah-1 SYNAPTOPHYSIN MMP9 VEGF 增殖 侵袭 凋亡 

分 类 号：R-33[医药卫生] R739.4