载脂蛋白B、性激素结合球蛋白及金属蛋白酶组织抑制剂-1在皖北地区原发性下腔静脉隔膜型布加综合征的临床意义  

Expression and clinical significance of Apolipoprotein B,sex hormone binding globulin and tissue inhibitors of metalloproteinase 1 in Budd-Chiari syndrome of inferior vena cava in northern Anhui

作　　者：张龙飞 高涌[1] 张懿刚 余朝文[1] 刘德朗 刘亚 陈世远[1] Zhang Longfei;Gao Yong;Zhang Yigang;Yu Chaowen;Liu Delang;Liu Ya;Chen Shiyuan(Department of Vascular Surgery,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233004,China;Department of General Surgery,Sichuan Province Dazhu County People’s Hospital,Dazhu 635100,China;Department of Hepatobiliary Surgery,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233004,China;Department of General Surgery,Bengbu First People’s Hospital,Bengbu 233000,China)

机构地区：[1]蚌埠医学院第一附属医院血管外科,233004 [2]四川省大竹县人民医院普外科,635100 [3]蚌埠医学院第一附属医院肝胆外科,233004 [4]蚌埠市第一人民医院普外科,233000

出　　处：《中华普通外科学文献（电子版）》2023年第4期252-256,共5页Chinese Archives of General Surgery(Electronic Edition)

基　　金：安徽省高校自然科学研究重点项目(KJ2020A0558);安徽省高校研究生科学研究项目(YJS20210538)。

摘　　要：目的探讨载脂蛋白B(ApoB)、性激素结合球蛋白(SHBG)及金属蛋白酶组织抑制剂-1(TIMP-1)在皖北地区原发性下腔静脉隔膜型布加综合征(BCS)患者血清中的表达情况。方法选取2021年9月至2022年4月蚌埠医学院第一附属医院16例确诊为原发性下腔静脉隔膜型BCS患者为试验组,8名健康成年人为对照组。应用酶联免疫吸附法检测ApoB、SHBG及TIMP-1在两组血清中含量;采用受试者工作特征(ROC)曲线分析评价差异蛋白对BCS的诊断效能;qPCR检测三个蛋白的基因在人脐静脉内皮细胞(HUVEC)中的表达丰度。结果两组血清ApoB、SHBG表达水平差异无统计学意义(t=0.93、1.12,P=0.36、0.27),试验组血清TIMP-1表达水平低于对照组(t=2.31,P<0.05);ROC曲线分析显示,TIMP-1的ROC曲线下面积(AUC)为0.75。qPCR提示ApoB、SHBG在HUVEC中呈低表达,TIMP-1表达丰度高。结论TIMP-1可能与皖北地区原发性下腔静脉隔膜型BCS具有相关性,可作为进一步研究的潜在血清生物标志物。Objective To investigate the expression and clinical significance of Apolipoprotein B(ApoB),sex hormone binding globulin(SHBG)and tissue inhibitors of metalloproteinase 1(TIMP-1)in serum of patients with primary inferior vena cava diaphragm type Budd-Chiari syndrome(BCS)in northern Anhui.Methods Sixteen patients diagnosed with primary inferior vena cava septal BCS in northern Anhui were selected as the experimental group and eight healthy adults were selected as the control group.The contents of ApoB,SHBG and TIMP-1 in serum of the two groups were detected by enzyme-linked immunosorbent assay(ELISA).Receiver operating characteristic(ROC)curve was used to evaluate the diagnostic efficiency of differential protein in BCS.qPCR was used to detect the expression abundance of the three protein genes in human umbilical vein endothelial cells(HUVEC).Results ELISA results showed that the expression levels of serum ApoB and SHBG were not statistically significant between the two groups(t=0.93,1.12,P=0.36,0.27),and the expression level of serum TIMP-1 in the experimental group was lower than that in the control group(t=2.31,P<0.05).ROC curve analysis showed that the area under curve(AUC)of TIMP-1 was 0.75.qPCR indicated that ApoB and SHBG were lowly expressed in HUVEC,while TIMP-1 expression was high.Conclusion TIMP-1 may be correlated with primary septal BCS of inferior vena cava in northern Anhui,and can be used as a potential serum biomarker for further study.

关 键 词：载脂蛋白B 性激素结合球蛋白 金属蛋白酶组织抑制剂-1 布加综合征 

分 类 号：R575[医药卫生—消化系统]