玉米秸秆纤维素降解菌的紫外-微波复合诱变及产酶条件优化  

Optimization of UV-Microwave Combined Induction and Enzyme Production Conditions of Cellulose-Degrading Bacteria for Maize Straw

作　　者：魏炜[1] 赵之璧 袁雅姝[1] WEI Wei;ZHAO Zhibi;YUAN Yashu(School of Municipal and Environmental Engineering,Shenyang Jianzhu University,Shenyang,China,110168)

机构地区：[1]沈阳建筑大学市政与环境工程学院,辽宁沈阳110168

出　　处：《沈阳建筑大学学报（自然科学版）》2023年第4期761-768,共8页Journal of Shenyang Jianzhu University：Natural Science

基　　金：国家自然科学基金项目(51908377)。

摘　　要：目的筛选出降解性能更好的玉米秸秆纤维素降解菌株,为秸秆生物降解提供理论依据。方法利用刚果红染色法对菌株筛选分离并鉴定;通过复合诱变,获得降解性更好的菌株;采用单因素及正交试验测定不同条件下的羧甲基纤维素(CMC)酶活力和滤纸(FPA)酶活力,确定最佳产酶条件。结果经复合诱变获得1株降解性较好的菌株SF3-3,降解率比未诱变菌株提升18.12%,比单独诱变菌株提升10.84%;最适合产酶条件为温度28℃、pH值7.0、接种量2%、转速130 r/min。结论紫外-微波复合诱变能更好地提升菌株对秸秆的降解性能,最佳的产酶条件能够实现降解性能的进一步提升。The corn straw cellulose degrading strains with preferable degradation performance were screened out to provide theoretical basis for straw biodegradation.The strains was screened,isolated and identified by Congo red staining method,and has better biodegradability through compound mutagenesis.The optimal enzyme production conditions were determined by the enzyme activity of carboxymethyⅠcellulose(CMC)and filter paper(FPA)under different conditions which were determined by single factor and orthogonal experiments.A strain SF3-3 with preferable biodegradability was obtained by compound mutagenesis,which was 18.12%higher than that by non mutagenesis and 21.68%higher than that by single mutagenesis;The optimal enzyme production conditions were temperature 28℃,pH 7.0,inoculation amount 2%,and rotation speed 130r·min^(-1).So the degradation performance of the strain for corn straw can be improved by UV microwave compound mutagenesis,and the optimal enzyme production conditions can further improve this performance.

关 键 词：秸秆降解菌 紫外 微波 复合诱变 

分 类 号：TU-023[建筑科学—建筑理论] X172[环境科学与工程—环境科学]