尿液细胞外囊泡circRNA分类器对高级别前列腺癌诊断价值的初步研究  

A preliminary study of the diagnostic value of urine extracellular vesicle circRNA classifier for prostate cancer

作　　者：王邦郁 陈晓鹏 唐国军 王佳妮[1] Bangyu Wang;Xiaopeng Chen;Guojun Tang;Jiani Wang(Department of Thyroid and breast,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China;Department of Urology,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China)

机构地区：[1]中山大学附属第三医院甲乳外科,广州510630 [2]中山大学附属第三医院泌尿外科

出　　处：《中华腔镜泌尿外科杂志（电子版）》2023年第4期339-342,共4页Chinese Journal of Endourology(Electronic Edition)

基　　金：国家自然科学基金(81502268)。

摘　　要：目的检验尿液细胞外囊泡环状RNA(circRNA)分类器(Ccirc)在PSA>2 ng/ml患者中的应用效能,进而评估其在高级别前列腺癌诊断中的潜在应用价值。方法收集23例高级别前列腺癌和17例低级别前列腺癌/前列腺增生患者尿液,运用第三代微滴数字PCR技术来测定他们尿液中的细胞外囊泡circRNA表达水平。以前列腺活体组织病理结果为金标准,利用受试者工作特征(ROC)曲线评估尿液细胞外囊泡circRNA分类器(Ccirc)和PSA诊断高级别前列腺癌的效果。结果高级别前列腺癌组和低级别前列腺癌/前列腺增生组Ccirc表达量分别为(7.78±1.04)copies/ml和(6.63±1.18)copies/ml,高级别前列腺癌组Ccirc表达量高于低级别前列腺癌/前列腺增生组(P=0.003);尿液细胞外囊泡circRNA分类器和PSA诊断高级别前列腺癌的ROC曲线下面积分别为0.757(95%CI 0.596~0.878)、0.586(95%CI 0.519~0.639)。结论尿液细胞外囊泡circRNA分类器(Ccirc)对诊断PSA>2 ng/ml的高级别前列腺癌患者具有良好的效能。Objective To examine the efficacy of the urine extracellular vesicle circRNA classifier(Ccirc)in patients with PSA>2 ng/ml to evaluate its potential application in prostate cancer diagnosis.Methods Twenty-three high-grade prostate cancer cases and 17 low-grade prostate cancer/prostatic hyperplasia cases were screened to measure extracellular vesicle circRNA expression in their urine using third-generation droplet digital PCR technology.The pathological results of prostate biopsy(biopsy)were used as judging criteria,and the effect of urine extracellular vesicle circRNA classifier(Ccirc)and PSA in the diagnosis of high-grade prostate cancer was evaluated using the receiver working characteristics(ROC)curve.Results The expression of Ccirc in the high-grade prostate cancer group and the low-grade prostate cancer/prostatic hyperplasia group were(7.78±1.04)copies/ml and(6.63±1.18)copies/ml,respectively,and the Ccirc expression in the high-grade prostate cancer group was higher than that in the low-grade prostate cancer/prostatic hyperplasia group(P=0.003).The area under the ROC curve for the diagnosis of high-grade prostate cancer by urine extracellular vesicle circRNA classifier and PSA was(95%CI 0.596-0.878)and 0.586(95%CI 0.419-0.739),respectively.Conclusion The urine extracellular vesicle circRNA classifier(Ccirc)has good efficacy in diagnosing high-grade prostate cancer patients with PSA>2 ng/ml.

关 键 词：前列腺癌 环状RNA 细胞外囊泡 诊断 

分 类 号：R737.25[医药卫生—肿瘤]