细胞器靶向型半胱氨酸荧光探针研究进展  

Research Progress of Fluorescent Probes for Cysteine Targeting Cellular Organelles

作　　者：刘飞冉 敬静 张小玲 Liu Feiran;Jing Jing;Zhang Xiaoling(School of Chemistry and Chemical Engineering,Beijing Institute of Technology,Beijing 100081;School of Medical Technology,Beijing Institute of Technology,Beijing 100081)

机构地区：[1]北京理工大学化学与化工学院,北京100081 [2]北京理工大学医学技术学院,北京100081

出　　处：《有机化学》2023年第6期2053-2067,共15页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(Nos.22174008,22177013,21974009,22274010)资助项目。

摘　　要：半胱氨酸作为一种重要的内源性小分子硫醇,参与众多生理过程,对细胞内氧化还原水平的调节和生物活性物质的合成具有重要意义.细胞器在生命过程中发挥着不可或缺的作用,不同细胞器具有各自独特的功能,而半胱氨酸在不同细胞器内的作用机制不尽相同.荧光探针的高灵敏度与原位检测能力提供了研究不同细胞器内半胱氨酸的方法,有助于更加全面了解半胱氨酸在不同亚细胞器中的作用机制.基于荧光探针所靶向的细胞器,总结了近年来报道的具有细胞器靶向性能的半胱氨酸荧光探针,并从其结构、响应机制、光谱学性质和生物应用等方面进行了比较和分析.As an important endogenous biothiol,cysteine is involved in various physiological processes.Besides,cysteine also plays an important role in the regulation of intracellular redox levels and the synthesis of bioactive substances.Cellular organelles all act an essential role in the life process,but different cellular organelles have their own unique functions,which makes the mechanism of cysteine in different organelles may be different.The high sensitivity and in situ analyze ability of fluorescent probes provide a method to monitor cysteine in different organelles,which is helpful to understand the mechanism of cysteine in physiological process more comprehensively.In this review,the recent reports on cysteine specific fluorescent probes are classified according to their targeted organelles.Besides,the property of those probes,including their structure,response mechanism,spectral properties and biological applications,is summarized.

关 键 词：半胱氨酸 细胞器 靶向定位 荧光探针 

分 类 号：Q244[生物学—细胞生物学] O657.3[理学—分析化学]