亚磁环境下小鼠生理及运动能力的适应性变化与天人相应理论  

Adaptive Changes of Physiological and Exercise Indexes of Mice in Hypomagnetic Environment and Theory of Correspondence between Nature and Human

作　　者：刘炳君 赫荣乔[2] 王焱冰 胡平东 王秀梅 刘缨 LIU Bingjun;HE Rongqiao;WANG Yanbing;HU Pingdong;WANG Xiumei;LIU Ying(School of Life Sciences,Beijing University of Chinese Medicine,Beijing 102488,China;State Key Laboratory of Brain and Cognitive Sciences,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China;National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]北京中医药大学生命科学学院,北京102488 [2]中国科学院生物物理研究所脑与认知国家重点实验室,北京100101 [3]中国科学院生物物理研究所大分子国家重点实验室,北京100101

出　　处：《世界中医药》2023年第15期2160-2165,2172,共7页World Chinese Medicine

基　　金：国家重点研发计划项目(2022YFA1604504);国家自然科学基金项目(31870840)。

摘　　要：目的:探索不同亚磁环境下小鼠生理及运动能力的变化规律,阐释天人相应理论的生物学基础,为未来载人航天生命保障中必须考虑的亚磁效应风险评估提供基础研究依据。方法:8周龄成年雄性C57BL/6小鼠60只,适应性饲养7天后,按照体质量随机分为亥姆霍兹亚磁组、亥姆霍兹对照组、坡莫合金亚磁组、坡莫合金对照组。采用有光照补偿式亥姆霍兹线圈系统(HCS)和无光照屏蔽式坡莫合金亚磁系统(PHS)构建的不同亚磁环境以及相应的地磁场环境进行小鼠长期连续培养,在1个月、3个月、12个月、24个月时,通过热板仪、跑步机、转棒等方法分别检测小鼠热痛觉敏感性,运动能力及身体协调能力的变化。结果:经过3个月亚磁场处理后,坡莫合金亚磁组的热痛觉敏感性明显高于亥姆霍兹对照组、坡莫合金对照组(P<0.01,P<0.05);亥姆霍兹亚磁组运动能力及身体协调能力明显低于其对照组(P<0.05),且坡莫合金对照组运动能力及身体协调能力明显小于亥姆霍兹对照组(P<0.05,P<0.01);处理时间12个月时,2种亚磁处理组在热痛觉敏感度上均高于其对照组(P<0.0001,P<0.01),且坡莫合金对照组小鼠的热痛觉敏感程度要显著高于亥姆霍兹对照组(P<0.05);2种亚磁处理组运动能力及身体协调能力均明显低于其对照组(P<0.05,P<0.01),坡莫合金亚磁组运动能力及身体协调能力明显低于亥姆霍兹亚磁组(P<0.001,P<0.05),坡莫合金对照组运动能力及身体协调能力明显低于亥姆霍兹对照组(P<0.001,P<0.01);24个月时,亚磁组小鼠各指标的差异下降,亥姆霍兹亚磁组和坡莫合金亚磁组饮水量明显高于其对照组(P<0.05,P<0.01),饮食量有上升趋势,坡莫合金亚磁组小鼠的空腹血糖水平较其他3组均明显下降(P<0.05,P<0.01,P<0.001),体质量差异无统计学意义。1个月亚磁处理小鼠各指标暂无差异。结论:长期亚磁场曝露会导致小鼠热痛觉敏感�Objective:To reveal the changes in the physiological indexes and exercise capacity of mice in different hypomagnetic fields,which is necessary for risk assessment and prevention of hypomagnetic effect in the life support system of manned space craft,and elucidate the mechanism underlying the traditional Chinese medicine theory of correspondence between nature and human.Methods:Sixty C57BL/6 mice of 8 weeks old received adaptive feeding for 7 days old and then randomized into Helmholtz hypomagnetic,Helmholtz control,Permalloy hypomagnetic,and Permalloy control groups according to the body weight.The mice were continuously treated in two hypomagnetic environments:the compensative Helmholtz coil system(HCS)with light and the non-light Permalloy-shielded hypomagnetic system(PHS),and in corresponding geomagnetic environments.The changes in the sensitivity to thermal pain,exercise capacity,and motor coordination of the mice were examined by hot plate apparatus,treadmill,and rotarod test,respectively,after 1,3,12,and 24 months.Results:After 3 months of treatment,the Permalloy hypomagnetic group had higher sensitivity to thermal pain than Helmholtz control group and Permalloy control group(P<0.01,P<0.05).The exercise capacity and motor coordination were lower in the Helmholtz hypomagnetic group than in the Helmholtz control group(P<0.05)and lower in the Permalloy control group than in the Helmholtz control group(P<0.05,P<0.01).After 12 months of treatment,the two hypomagnetic treatment groups had higher sensitivity to thermal pain than the control groups(P<0.0001,P<0.01),and the Permalloy control group had higher sensitivity to thermal pain than the Helmholtz control group(P<0.05).The exercise capacity and motor coordination of the two hypomagnetic groups were lower than those in the control groups(P<0.05,P<0.01).Moreover,the exercise capacity and motor coordination in the Permalloy hypomagnetic group were lower than those in the Helmholtz hypomagnetic group(P<0.001,P<0.05),and they were lower in the Permalloy control gr

关 键 词：亚磁场 小鼠 运动能力 身体协调能力 热痛觉敏感性 生理 曝露条件 亥姆霍兹线圈系统 坡莫合金亚磁系统 

分 类 号：R22[医药卫生—中医基础理论]