Quality Standard of Barberry Branches  

作　　者：Dongmei SHA Liniu SHAMA Wenbing LI Xiaoyong HE Bin HE Yanfei HUANG Xinjia YAN Yuan LIU 

机构地区：[1]College of Pharmacy,Southwest Minzu University,Chengdu 610041,China [2]Sichuan Provincial Engineering Laboratory of Protection and Utilization of Qiang and Yi Medicinal Resources,Chengdu 610225,China [3]Key Laboratory of Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization of National Ethnic Affairs Commission,Chengdu 610225,China [4]Institute of Qinghai-Tibetan Plateau,Southwest Minzu University,Chengdu 610225,China

出　　处：《Medicinal Plant》2023年第4期41-45,共5页药用植物：英文版

基　　金：Supported by the National Key Research and Development Plan(2018YFC-06101);Sichuan Provincial Natural Science Foundation(2022NSFSC1605);Traditional Chinese Medicine(Ethnic Medicine)Standard Improvement of Sichuan Medical Products Administration(510201202102305);Leading Talents Support Plan of National Ethnic Affairs Commission in 2022;Special Project for the University-Level Innovation Team in the Fundamental Research Funds for the Central Universities(ZYN2022067).

摘　　要：[Objectives] To establish the quality standard of barberry branches. [Methods] Microscopic identification and thin layer identification were used to qualitatively identify barberry branches. Berberine content was determined by HPLC method, and the content of water, total ash, acid insoluble ash and extract was detected according to the method of the Chinese Pharmacopoeia (2020 edition). [Results] The microscopic identification showed that the features were obvious, and stone cells, cork cells, epidermal cells, stomata, fibers and catheter with reticulated pores could be found. Berberine was detected in barberry branches by thin layer chromatography, and the characteristic spots were separated clearly. Moisture, total ash, and acid insoluble ash content shall not exceed 13%, 6%, and 3%, respectively, and extract content shall not be less than 10%. Berberine hydrochloride (C_(20)H_(18)ClNO_(4)) should be calculated in the branches of Berberis wilsonae Hemsley and Berberis aggregata C. K. Schneid., and berberine (C_(20)H_(17)NO_(4)) content should not be less than 0.05%. The linear relationship was good in the range of 0.002-0.240 mg/mL ( R^(2)=0.999 5). The average recovery was 89.63%, and RSD was 5.28%. [Conclusions] The method was simple, accurate and reproducible, and can be used for the quality control of barberry branches.

关 键 词：Barberry branches BERBERINE BERBERIS Berberis wilsonae Hemsley Berberis aggregata C.K.Schneid. TLC HPLC 

分 类 号：R284[医药卫生—中药学]