花生蔗糖含量与蛋白质和含油量的相关性分析及蔗糖含量QTL定位  

Correlation analysis of sucrose content with protein and oil content and QTL mapping of sucrose content in peanut

作　　者：郭建斌[1] 成良强 李威涛 刘念[1] 罗怀勇[1] 丁膺宾 喻博伦 陈伟刚[1] 黄莉[1] 周小静[1] 雷永[1] 廖伯寿[1] 姜慧芳[1] GUO Jian-Bin;CHENG Liang-Qiang;LI Wei-Tao;LIU Nian;LUO Huai-Yong;DING Ying-Bin;YU Bo-Lun;CHEN Wei-Gang;HUANG Li;ZHOU Xiao-Jing;LEI Yong;LIAO Bo-Shou;JIANG Hui-Fang(Oil Crops Research Institute,China Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops,the Ministry of Agriculture and Rural Affairs,Wuhan 430062,Hubei,China;Guizhou Oil Research Institute,Guizhou Academy of Agricultural Sciences,Guiyang 550006,Guizhou,China)

机构地区：[1]中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [2]贵州省农业科学院油料研究所,贵州贵阳550006

出　　处：《作物学报》2023年第10期2698-2704,共7页Acta Agronomica Sinica

基　　金：贵州省科技计划项目(黔科合基础-ZK[2022]一般290);农业农村部油料作物生物学与遗传育种重点实验室开放课题项目(KF2020008);财政部和农业农村部国家现代农业产业技术体系建设专项(花生,CARS-13-种质资源评价)资助。

摘　　要：随着食用型花生市场规模的日益扩大,食用花生的品质特性备受关注。甜味是影响食用花生风味和口感的重要指标,而花生甜味主要来源于蔗糖。提高蔗糖含量是培育食用型花生的关键,定位蔗糖含量相关的QTL,将为花生高糖分子育种奠定基础。但提高蔗糖可能对蛋白质和含油量有影响。本研究以徐花13和中花6号为亲本构建的重组自交系群体(RIL)为材料,分析了蔗糖与蛋白质和含油量的关系,并对蔗糖含量进行了QTL定位。结果表明,蔗糖含量与蛋白质含量无显著相关性,而与含油量呈极显著负相关;RIL群体中蔗糖含量变异广泛,变异范围为14.33~61.42mg g^(-1);筛选出2份高蛋白低脂肪中等含糖量的材料。通过该群体的遗传连锁图,在3个环境下共检测到10个QTL,分布在6条染色体上,贡献率为4.56%~12.25%。其中,qSUCA07能在3个环境下被重复检测到。本研究为蔗糖含量精细定位和培育性状优良的食用型花生品种提供了重要信息。With the increasing size of edible peanuts market,the quality characteristics of edible peanuts have attracted much at-tention.Sweetness is an important indicator affecting the flavor and taste of edible peanuts,and it mainly comes from sucrose.Increasing sucrose content is the key to the edible peanuts cultivation,and QTL mapping for sucrose content will lay a founda-tion for molecular breeding of high sucrose content.However,the increase of sucrose content may affect protein and oil content.In this study,a recombinant inbred line population derived from the cross of Xuhua 13 and Zhonghua 6 was developed.The rela-tionships between sucrose and protein as well as oil content were investigated,and QTLs for sucrose content were mapped.The results showed that sucrose content was not significantly correlated with protein content but there was extremely significantly negatively correlated with the content.Sucrose content presented board phenotypic variations among the RILs,ranging from 14.33 mg g^(-1) to 61.42 mg g^(-1).Two lines with high protein,low oil content,and medium sucrose content were observed.Ten QTLs on six chromosomes were detected with 4.56%–12.25%of phenotypic variation explained(PVE)among which qSUCA07 could be repeatedly detected in three environments.The results provide an important information for QTLs fine mapping of sucrose content and cultivating edible peanut varieties with excellent traits.

关 键 词：花生 含油量 蛋白质 蔗糖 QTL 

分 类 号：S565.2[农业科学—作物学]