中高温鸡粪厌氧消化微生物调节机制对比及耐热机理研究  

Comparison of microbial regulation mechanisms and heat-resistant mechanism of anaerobic digestion of chicken manure at medium and high temperature

作　　者：王晓鑫 王建 马峰 张延平 王志鹏 党小燕 姚赛 朱彤[2] 王有昭[2] 李旭[2] WANG Xiaoxin;WANG Jian;MA Feng;ZHANG Yanping;WANG Zhipeng;DANG Xiaoyan;YAO Sai;ZHU Tong;WANG Youzhao;LI Xu(Baotou Renewable Water Resources and Sewage Treatment Co.,LTD,Baotou 110168,China;School of Mechanical Engineering and Automation,Northeastern University,Shenyang 110819,China)

机构地区：[1]包头市再生水资源及污水处理有限责任公司,包头014000 [2]东北大学机械工程与自动化学院,沈阳110168

出　　处：《环境保护科学》2023年第4期74-84,共11页Environmental Protection Science

基　　金：国家重点研发计划课题(2020YFC1806402);沈阳市重大关键核心技术攻关项目(20-202-4-37)。

摘　　要：温度对厌氧消化系统内微生物调节机制的影响尚不清楚,通过对比中高温厌氧消化实验,分析了其各自微生物调节机制以及高温厌氧消化耐热机理。结果表明,在底物浓度相同的情况下高温厌氧消化比中温提前6天达到产甲烷峰值,高温条件能够缩短厌氧消化反应周期,更有利于有机物的水解酸化和甲烷的生成;Defluviitoga作为高温厌氧消化过程水解阶段优势菌种,对高温环境具有较好的抗逆性,可将大部分多糖类物质当作电子受体,并降解为醋酸盐、H_(2)和CO_(2);Methanosarcina作为中高温厌氧消化产甲烷阶段优势菌种,能够适应中温高温两种不同环境,且可利用所有甲烷代谢途径(食乙酸、食氢、食甲基化合物),产甲烷潜力巨大。The influence of temperature on the microbial regulation mechanism in the anaerobic digestion system is unclear.The microbial regulation mechanisms and the mechanism of heat resistance of high-temperature anaerobic digestion by comparing the anaerobic digestion experiments at medium and high temperatures were analyzed.The results showed that under the condition of the same substrate concentration,high-temperature anaerobic digestion reached the peak of methane production 6 days earlier than medium-temperature,and high-temperature conditions could shorten the reaction cycle,which was more conducive to the hydrolysis and acidification of organic matter and the formation of methane.Defluviitoga,as the dominant strain in the hydrolysis stage of the high-temperature anaerobic digestion process,had good stress resistance to high-temperature and could use most polysaccharides as electron acceptors and degrade them into acetate,H_(2) and CO_(2).Methanosarcina,as the dominant strain in the methanogenic stage of medium-high temperature anaerobic digestion,could adapt to two different environments,use all methane metabolic pathways(eating acetic acid,hydrogen,and methyl compounds),and has great potential for methane production.

关 键 词：消化温度 厌氧消化 菌群丰度 微生物多样性 耐热机制 

分 类 号：X705[环境科学与工程—环境工程]