基于GEO数据库筛选舌鳞状细胞癌顺铂耐药的关键基因及生物信息学分析  

Screening the key genes of cisplatin resistance in tongue squamous cell carcinomaby bioinformatics analysis based on GEO database

作　　者：苏萌 张钊银 姚金光 Su Meng;Zhang Zhaoyin;Yao Jinguang(School of Stomatology,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)

机构地区：[1]右江民族医学院口腔医学院,广西百色533000

出　　处：《右江民族医学院学报》2023年第4期557-562,共6页Journal of Youjiang Medical University for Nationalities

基　　金：国家自然科学基金项目(81660495);广西研究生教育创新计划项目(YCSW2022459)。

摘　　要：目的通过生物信息学方法筛选舌鳞状细胞癌顺铂耐药的关键基因。方法从GEO数据库下载GSE111585和GSE115119数据集,利用limma包进行差异分析,与PRGs取交集后获取DEGs。利用R语言对DEGs进行GO功能富集及KEGG通路富集分析。String数据库构建PPI网络,在Cytoscape中进行可视化,基于Degree拓扑分析算法筛选Hub基因。根据Wilcox.test法分析Hub基因与顺铂耐药性的联系。结果①共筛选出32个DEGs。②GO富集分析显示DEGs主要参与刺激反应调节、信号受体结合等功能;KEGG富集分析显示DEGs富集在癌症通路和人T细胞白血病病毒Ⅰ型感染信号通路。③筛选出FN1、SOX2和COL1A13个Hub基因,其中COL1A1具有成为潜在生物靶点的潜力。结论COL1A1可能是舌鳞状细胞癌顺铂耐药的潜在作用靶点。Objective To screen the key genes of cisplatin resistance in tongue squamous cell carcinoma by bioinformatics methods.Methods The GSE111585 and GSE115119 datasets were downloaded from the GEO database,differential analysis was performed using the limma package,and the DEGs were acquired after adopting the intersection with PRGs.The GO functional enrichment and KEGG pathway enrichment analysis of DEGs were performed by R language.The PPI network was constructed using the String database,and was visualized in Cytoscape.Hub genes were selected based on the Degree topological analysis algorithm.The relationship between Hub genes and cisplatin resistance was analyzed with the Wilcox.test method.Results①A total of 32 DEGs were screened.②The GO enrichment analysis showed that the DEGs were main involved in functions such as stimulus response regulation,signal receptor binding.KEGG enrichment analysis showed that the DEGs were enriched in the cancer pathways and typeⅠinfection signaling pathway of human T-cell leukemia virus.③FN1,SOX2,and COL1A1 were identified as 3 hub genes,with a potential biological target for COL1A1.Conclusion COL1A1 may be a potential target for cisplatin resistance in tongue squamous cell carcinoma.

关 键 词：舌肿瘤 生物信息学 顺铂 耐药性 靶点 

分 类 号：R739.86[医药卫生—肿瘤]