Testin基因对鼻咽癌5-8F细胞裸鼠移植瘤放疗敏感性的影响  

Effect of Testin Gene on Radiosensitivity of Nasopharyngeal Cancer 5-8F Cells

作　　者：钟准 费永光 ZHONG Zhun;FEI Yongguang(Department of Otolaryngology,Hubei NO.3 People’s Hospital of Jianghan University,Wuhan 430033,China;Department of Otolaryngology,Xinzhou District People's Hospital,Wuhan 431400,China)

机构地区：[1]江汉大学附属湖北省第三人民医院耳鼻咽喉科,武汉430033 [2]武汉市新洲区人民医院耳鼻咽喉科,武汉431400

出　　处：《肿瘤防治研究》2023年第8期767-771,共5页Cancer Research on Prevention and Treatment

基　　金：武汉市卫生健康委科研基金资助项目(WX19Y02)。

摘　　要：目的探讨Testin基因(TES)对鼻咽癌5-8F细胞裸鼠移植瘤放疗敏感性的影响。方法将鼻咽癌5-8F细胞分为两组:未转染组(正常的5-8F细胞)和TES组(高表达TES的5-8F细胞),经过0、2、4、6、8 Gy放射剂量点照射后,进行细胞克隆形成实验,绘制生存曲线。为建立鼻咽癌5-8F细胞裸鼠移植瘤模型,将24只BALB/c Nude裸鼠随机均分为四组:未转染组(注射5-8F细胞液)、TES组(注射高表达TES的5-8F细胞液)、照射组(注射5-8F细胞液+局部照射)、TES照射组(注射高表达TES的5-8F细胞液+局部照射),每隔3天测量移植瘤长短直径,计算肿瘤体积并绘制移植瘤生长曲线,1月后处死小鼠,取肿瘤块称质量,流式细胞仪检测各组移植瘤中细胞凋亡情况。结果与未转染组比较,TES组细胞的存活率明显降低(P<0.01)。未转染组、TES组、照射组、TES照射组瘤体生长速度依次减慢、瘤体质量依次降低、细胞凋亡率依次增加。以TES照射组瘤体生长速度最慢、质量下降最大、细胞凋亡率最高,组间多重比较差异均有统计学意义(P<0.05)。结论Testin基因能有效提高体外培养的鼻咽癌5-8F细胞的放疗敏感性。Objective To investigate the effect of TES gene on the radiosensitivity of nasopharyngeal carcinoma 5-8F cells.Methods Specimens of 5-8F cells(unprocessed group)and 5-8F cells with high TES expression(TES group)were irradiated at 0,2,4,6,and 8 Gy radiation dose points.Cell clone formation experiment was conducted to draw the survival curve.Twenty-four BALB/c nude mice were randomly divided into four groups:nontransfection group,TES group,irradiation group,and TES irradiation group.A nude mouse model of nasopharyngeal carcinoma 5-8F cells was established.The length and diameter of the transplanted tumor were measured every three days,the tumor volume was calculated,and the growth curve of the transplanted tumor was drawn.After the mice were killed one month later,the tumor block was taken and weighed.The apoptosis of the transplanted tumor cells in each group was detected by flow cytometry.Results Compared with that in the unprocessed group,the survival rate of cells in the TES group was significantly lower(P<0.01).The tumor growth rate and tumor mass of all four groups decreased in turn,while the apoptosis rate increased in turn.The TES irradiation group had the slowest tumor growth rate,greatest decrease in tumor weight,and highest apoptosis rate among the four groups.Multiple comparison revealed statistically significant differences between the groups(P<0.05).Conclusion The testin gene can effectively improve the radiosensitivity of nasopharyngeal carcinoma 5-8F cells cultured in vitro.

关 键 词：Testin基因 鼻咽癌 放疗敏感性 

分 类 号：R739.63[医药卫生—肿瘤]