小鼠烟雾吸入性损伤模型的建立与评估  

Establishment and evaluation of a novel smoke inhalation injury model in mice

作　　者：曲毅睿 柴家科[2] 胡方超 刘甜 迟云飞 孙冉 QU Yirui;CHAI Jiake;HU Fangchao;LIU Tian;CHI Yunfei;SUN Ran(Graduate School,Chinese PLA General Hospital,Beijing 100853,China;Burn Institute,the Fourth Medical Center,Chinese PLA General Hospital,Beijing 100048,China)

机构地区：[1]解放军总医院研究生院,北京100853 [2]解放军总医院第四医学中心全军烧伤研究所,北京100048

出　　处：《解放军医学院学报》2023年第6期678-684,共7页Academic Journal of Chinese PLA Medical School

基　　金：军委后勤保障部重大项目(AWS15J003,ALB19J001)。

摘　　要：背景吸入性损伤最常见为高温、有毒烟雾导致的损伤,然而关于其致病机制的研究仍不明确。目的研发一种简便易得的新型小鼠烟雾吸入性损伤模型,观察小鼠生理状态、组织病理学变化以及炎性因子改变。方法160只C57BL/6小鼠随机选取20只作为对照组,其余140只小鼠随机分为4组。采用自制烟雾致伤装置,以胶合板和松木屑作为发烟材料,实验组分别进行10 min、15 min、20 min、25 min烟雾吸入致伤,对照组小鼠不经烟雾吸入。观察烟雾吸入性损伤后小鼠行为状态、生理表现以及72 h生存变化情况;各组小鼠处死后取肺组织,观察其病理改变、干湿重改变及检测组织细胞内蛋白和炎性因子含量。结果致伤10 min、15 min、20 min、25 min后72 h生存率分别为100%、83%、54%和0,其中25 min致伤组小鼠在伤后12 h内全部死亡。与对照组相比,各致伤组小鼠伤后出现呼吸急促、活动减少,随后部分自行恢复至正常状态,部分进食进水减少,状态萎靡;主要观察致伤20 min组小鼠肺部病理组织,可见炎性细胞浸润,肺泡结构破坏,肺泡隔增厚;干湿重显示有肺水肿出现,伤后12 h和24 h小鼠肺组织匀浆炎性因子检测白细胞介素(interleukin,IL)-1β、IL-6和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)与对照组相比显著上升,IL-10显著下降(P均<0.05)。结论吸入20 min由胶合板和松木混合燃烧产生的烟雾可以导致C57BL/6小鼠中重度吸入性损伤,伤后实验动物的生理表现和组织病理结果与临床病情相符。Background Inhalation injury is most commonly caused by heat and toxic fumes,but studies on its pathogenic mechanisms remain elusive.Objective To develop a simple and easily available new mouse model of smoke inhalation injury,and observe the changes in the physiological status,histopathological and inflammatory factors of mice.Methods Of the 16057BL/6 mice,20 mice were randomly selected as the control group,the rest of the 140 mice were randomly divided into 4 groups.Smoke inhalation injury was performed for 10,15,20,and 25 min using a self-made smoke injury device with plywood and pine chips as smoke materials,respectively,the control group received no special treatment,and other treatments were the same as those in each smoke inhalation group.The behavioral status,physiological performance,and 72-hour survival changes in mice after smoke inhalation injury were observed.Lung tissues were taken from mice in each group after sacrifice to observe the pathological changes,dry and wet weight changes,and detect the contents of intracellular proteins and inflammatory factors in tissue cells.Results The survival rates at 72 hours post-inhalation injury for the above 10 min-,15 min-,20 min-,and 25 min-smoke-inhalation injury groups were 100%,83%,54%,and 0,respectively;and all the mice in the 25 minutes-smoke-inhalation injury group died within 12 hours after injury.Except for the control group,tachypnea and decreased activity were immediately observed after smoke inhalation injury in the live mice of each group and these abnormal signs subsided several hours later.Some mice in the experimental group showed loss of appetite and malaise.Inflammatory cell infiltration,alveolar structure destruction,and alveolar septum thickening were observed in the lung pathological tissues of mice in the 20 min smoke-inhalation injury group,and the dry and wet weight ratio showed the appearance of pulmonary edema.Interleukin-1β,6(IL-1β,IL-6)and tumor necrosis factor-α(TNF-α)detected by inflammatory factors in lung homogenates of mice

关 键 词：烧伤 烟雾吸入性损伤 C57BL/6小鼠 肺损伤动物模型 

分 类 号：R-332[医药卫生]