沙田柚种子黄酮类物质半仿生提取及体外抗氧化能力研究  

Determination of Flavonoids Antioxidant Activities of Semi-bionic Extract from Seeds of Citrus Maxima

作　　者：乐晶 林怡 邓燕慧 骆松涛 何泽标 刘惠娜[1] Le Jing;Lin Yi;Deng Yanhui;Luo Songtao;He Zebiao;Liu Huina(School of Life Science,Jiaying University,Meizhou 514015,China)

机构地区：[1]嘉应学院生命科学学院,广东梅州514015

出　　处：《广东化工》2023年第14期44-46,103,共4页Guangdong Chemical Industry

基　　金：广东省科技创新战略专项资金“攀登计划”专项资金(pdjh2021b0468)。

摘　　要：本试验采用超声波辅助,半仿生法提取沙田柚(Citrus maxima)种子黄酮类物质,并评价其抗氧化能力。沙田柚种子中类黄酮化合物有11类,数量和含量占比例较高是黄酮醇类。用模拟酸性,中性和碱性环境的溶液提取,得到类黄酮物质含量与70%甲醇提取含量没有显著差异。半仿生法提取液具有较高的Fe^(3+)还原能力、DPPH清除率和羟基自由基清除率,分别具有类黄酮浓度正相关效应。柚种子提取物具有一定的抗氧化活性,实践中可以用半仿生法提取代替有机溶剂提取柚种子活性成分,并且可以重点关注含量较多的黄酮醇类物质。Ultrasonic-Assisted Semi-Bionic Extraction of Flavonoids from Citrus maxima seeds and evaluated their antioxidant capacity.There are 11 classes of flavonoids in the seeds,with a higher proportion of flavonols.Using solutions that simulated acidic,neutral and alkaline environments,the flavonoid content was not significantly different from 70%methanol.and had high Fe^(3+)reduction capacity,DPPH clearance and hydroxyl radical clearance,with positive concentration correlation effect.Citrus maxima seeds extract had some antioxidant activity.In practice,semi-biSemi-Bionic Extraction can be used instead of organic solvent to extract the active ingredients,and Should focus on the relatively high content of flavonols.

关 键 词：沙田柚 种子 半仿生 抗氧化能力 类黄酮 

分 类 号：Q946.82[生物学—植物学]