瑶药白背清风藤三萜类化学成分及降血糖活性  

Chemical Constituents of Triterpenoidsfrom Sabia Discolor Dunnandwith Hypoglycemic Activity

作　　者：周文景 黄艳[2] 胡筱希 袁健童 宋志钊[2] 刘布鸣[2] 李力 ZHOU Wen-jing;HUANG Yan;HU Xiao-xi;YUAN Jian-ong;SONG Zhi-zhao;LIU Bu-ming;LI Li(School of Chemistry and Materials,Nanning Normal University,Nanning 530100,China;Guangxi Key Laboratory of Tradtitional Chinese Medicine Quality Standards,Guangxi Institute of Chinese Traditional Medical&Pharmaceutical Science,Nanning 530022,China)

机构地区：[1]南宁师范大学化学与材料学院,广西南宁530100 [2]广西中医药研究院广西中药质量标准研究重点实验室,广西南宁530022

出　　处：《南宁师范大学学报（自然科学版）》2023年第2期73-81,共9页Journal of Nanning Normal University：Natural Science Edition

基　　金：广西重点研发计划“中药材外源性污染物快速检测技术研究”(桂科AB20238017);广西科技厅重点研发计划;广西中药质量标准研究重点实验室自主研究课题(桂中重自202003)。

摘　　要：为研究白背清风藤(Sabia discolor Dunn)的化学成分及其对降血糖活性的体外抑制作用。该文采用硅胶柱层析、凝胶柱层析和半制备液相色谱等方法对白背清风藤进行分离纯化,运用^(1)H NMR、^(13)C NMR和MS等波谱技术对化合物进行结构鉴定;并对所有化合物的α-葡萄糖苷酶的体外抑制作用进行测定。结果表明:(1)从白背清风藤干燥枝叶中分离得到16个五环三萜类化合物,分别鉴定为:3α-羟基-齐墩果酸甲酯(1)、二羟基蒲公英烷(2)、(20S)-3-oxo-20-hydroxytaraxastane(3)、白桦脂醇(4)、20-taraxastene-3α,28-diol(5)、sabiadiscolor A(6)、1-oxo-erythrodiol(7)、3-epi-betulin(8)、蒲公英烷-3β,20α-二醇(9)、1β,2α,3β-trihydroxylup-20(29)-ene(10)、paracaseolin A(11)、sabiadiscolor C(12)、3β-hydroxyolean-12-en-28-oic acid(13)、乌苏酸(14)、olean-2,12-dien-1-one(15)和juglangenin A(16)。其中化合物1、8、10、11、12、13和15为首次从该植物中分离得到。(2)药理活性结果表明,化合物5、8和11均对α-葡萄糖苷酶具有显著的抑制活性,IC_(50)值分别为(16.73±1.23)、(20.06±1.39)和(17.87±3.49)μmol/L,抑制活性与阳性药阿卡波糖相当(IC_(50)=26.23±1.37μmol/L)。该研究结果丰富了白背清风藤降血糖的活性物质基础,为其进一步开发利用提供了科学依据。In order to explore the chemical constituents of Sabia discolor Dunnand their inhibitory effects on hypoglycemic activity in vitro.The chemical constituents of S.discolor Dunn were isolated and purified by silica gel column chromatography,Sephadex LH-20 column chromatography andsemi-preparative HPLC,and their structures were identified by means of various spectroscopic techniques such as ^(1)H NMR,^(13)C NMR and MS.An in vitro screening model was employed to test theα-glucosidase activity of obtained compounds.The results showed as follows:(1)Sixteen compounds were isolated from S.discolor Dunn and identified as3α-hydroxy-oleanolic acid methyl ester(1),dihydroxy-dandelane(2),(20S)-3-oxo-20-hydroxytaraxastane(3),betulinol(4),20-taraxastene-3α,28-diol(5),sabiadiscolor A(6),1-oxo-erythrodiol(7),3-epi-betulin(8),dandelane-3β,20α-diol(9),1β,2α,3β-trihydroxyl-20(29)-ene(10),paracaseolin A(11),sabiadiscolor C(12),3β-hydroxyolean-12-en-28-oic acid(13),ursolic acid(14),olean-2,12-dien-1-one(15)and juglangenin A(16).Compounds 1,8,10,11,12,13 and 15 were isolated from this plant for the first time.(2)The results of activity test showed that 20-taraxastene-3α,28-diol(5),3-epi-betulin(8)and paracaseolin A(11)had good inhibitory effects onα-glucosidase in vitro,with IC_(50) values of(16.73±1.23),(20.06±1.39),and(17.87±3.49)μmol/L,respectively,whose activitieswere equivalent to the positive drug acarbose(IC_(50)=26.23±1.37μmol/L).This study enrich the material basis of hypoglycemic activity of S.discolor Dunn,providing scientific basis for its further development and utilization.

关 键 词：清风藤属 白背清风藤 三萜类 化学成分 Α-葡萄糖苷酶 

分 类 号：Q946[生物学—植物学]