康莱特注射液对人胆管癌细胞RBE和HCCC9810生长、凋亡、迁移的影响  

The Effects of Kanglaite Injection on the Growth,Apoptosis and Migration of Human Cholangiocarcinoma Cancer Cells RBE and HCCC9810

作　　者：史亚波 周梦娜 刘华兵 殷俊翔 曹振军 龚帅昌 SHI Ya-bo;ZHOU Meng-na;LIU Hua-bing;YIN Jun-xiang;CAO Zhen-jun;GONG Shuai-chang(School of Medicine,Nanchang University,Jiangxi,Nanchang330006;Jiangxi Provincial People's Hospital,Nanchang330006;The First Affiliated Hospital of Nanchang Medical College,Jiangxi,Nanchang330006)

机构地区：[1]南昌大学医学院,江西南昌330006 [2]江西省人民医院,南昌330006 [3]南昌医学院第一附属医院,江西南昌330006

出　　处：《实用中西医结合临床》2023年第11期1-5,13,共6页Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine

基　　金：江西省中医药管理局资助项目(编号:2020B0362)。

摘　　要：目的:探讨康莱特注射液对人胆管癌细胞RBE和HCCC9810生长、凋亡、迁移的影响。方法:使用康莱特注射液处理人胆管癌细胞RBE和HCCC9810,运用CCK-8法检测康莱特注射液对人胆管癌细胞RBE和HCCC9810的增殖情况,流式细胞仪检测两者的凋亡率,Transwell实验检测两者的迁移能力,通过Western Blot检测两者信号通路中关键蛋白Caspase-3、Bcl-2和Bax的表达情况。结果:较之空白对照组,随着康莱特药物作用时间的延长,两者细胞的生长增殖程度逐渐受到抑制(P<0.05),在作用96 h时达到较好抑制状态;Transwell实验结果显示,人胆管癌细胞RBE和HCCC9810经康莱特处理后迁移数量减少(P<0.05);实验组人胆管癌细胞RBE和HCCC9810凋亡率明显增加(P<0.05),两者信号通路中关键蛋白Caspase-3、Bax蛋白表达明显上调(P<0.05),Bcl-2蛋白表达下调(P<0.05)。结论:康莱特注射液可以抑制人胆管癌细胞RBE和HCCC9810的生长和迁移能力,促进其凋亡,机制可能与其能上调凋亡信号通路关键蛋白Caspase-3、Bax和下调Bcl-2蛋白表达有关。Objective:To investigate the effects of Kanglaite injection on the growth,apoptosis and migration of human cholangiocarcinoma cells RBE and HCCC9810.Methods:Kanglaite injection was used to treat human cholangiocarcinoma cells RBE and HCCC9810,the proliferation of Kanglaite injection on human cholangiocarcinoma cells RBE and HCCC9810 were detected by CCK-8 method,the apoptosis rate of both were detected by flow cytometry,and the migration ability of both were detected by Transwell assay.The expressions of key proteins Caspase-3,Bcl-2 and Bax in both signaling pathways were detected by Western blot.Results:Compared to the control group,the growth and proliferation degree of both cells were gradually inhibited while the prolongation of Kanglaite action time(P<0.05),and reached a better inhibitory state after 96 hour of treatment.Transwell assay showed that the number of migration of human bile duct cancer cells RBE and HCCC9810 decreased after Kanglaite treatment(P<0.05).The apoptosis rate of RBE and HCCC9810 of bile duct cancer cells in the experimental group was significantly increased(P<0.05),the expressions of key proteins Caspase-3 and Bax in both signaling pathways were significantly up-regulated(P<0.05),and the expression of Bcl-2 protein was down-regulated(P<0.05).Conclusion:Kanglaite injection can inhibit the growth and migration of RBE and HCCC9810 of human cholangiocarcinoma cells,and promote their apoptosis.The mechanism may be related to the up-regulation of apoptosis signaling pathway key proteins Caspase-3 and Bax and down-regulation of Bcl-2 protein expression.

关 键 词：人胆管癌细胞 康莱特注射液 生长 凋亡 迁移 

分 类 号：R735.8[医药卫生—肿瘤]