CEACAM1在肺腺癌中表达及免疫浸润分析  

Ex pression of CEACAM1 in adenocarcinoma of the lung and analysis of its immune infiltration

作　　者：陈海欣 陈平[2] 陈婷婷[2] Chen Haixin

机构地区：[1]浙江中医药大学第一临床医学院,310053 [2]浙江中医药大学附属第一医院,310003

出　　处：《浙江临床医学》2023年第7期956-960,共5页Zhejiang Clinical Medical Journal

基　　金：浙江省医药卫生科技项目(2022KY223、2021PY056、2020KY197);浙江省中医药科技计划项目(2023ZL398、2020ZA041)。

摘　　要：目的探讨癌胚抗原相关细胞黏附分子1(CEACAM1)在肺腺癌(LUAD)中的表达差异及免疫浸润相关性分析。方法通过R软件包分析TCGA-LUAD数据库中肿瘤组织及癌旁组织中CEACAM1 mRNA表达差异;应用HPA数据库分析CEACAM1在肿瘤组织和正常肺组织的免疫组化结果差异;采用KM生存曲线分析不同病理分期的LUAD患者CEACAM1高低表达组总生存率(OS)差异;免疫浸润相关性分析CEACAM1与肿瘤细胞中免疫细胞浸润程度的相关性;单基因共表达分析CEACAM1与多种免疫检查点的共表达情况;基因富集分析(GSEA)鉴定LUAD中与CEACAM1相关的信号通路。结果较癌旁组织,肿瘤组织中CEACAM1 mRNA高表达(P<0.05);免疫组化显示CEACAM1在肿瘤组织中荧光强度强,而正常肺组织弱;KM生存曲线显示LUAD患者中CEACAM1高表达组OS偏低,尤其在I期和II患者中OS偏低更显著(P<0.05);免疫浸润相关性分析显示CEACAM1与肿瘤组织中滤泡辅助T细胞(TFH)、活化的树突状细胞(aDC)、调节性T细胞(Treg)、T细胞、嗜酸性粒细胞、B细胞、Th1细胞以及幼稚的树突状细胞(iDC)浸润程度呈正相关(P<0.05),其中与TFH相关性最显著;免疫检查点分子共表达分析显示CEACAM1与SIGLEC15、TIGIT、CD274、HAVCR2、PDCD1、CTLA4和LAG3的表达呈正相关(P<0.05);GSEA通路富集分析发现CEACAM1与Rho GTP酶信号传导、M期有丝分裂、NABA分泌因子、DNA修复以及氨基酸及其衍生物的代谢通路相关。结论CEACAM1有可能成为早期LUAD不良预后标志物,并且与免疫细胞浸润密切相关;CEACAM1作为免疫检查点,可以通过与其他免疫检查点共表达调节免疫细胞抗肿瘤功能。Objective To analyze the expression of CEACAM1 in lung adenocarcinoma(LUAD)and to analyze the correlation of immune infiltration.Methods The differential expression of CEACAM1 mRNA in tumor tissues and adjacent tissues in TCGA-LUAD database was analyzed by R package.HPA database was used to analyze the immunohistochemical results of CEACAM1 in tumor tissues and the normal lung tissues.KM survival curve was used to analyze the difference of the overall survival rate(OS)of the CEACAM1 high and low expression groups in different pathological stages.The correlation between CEACAM1 and the degree of immune cell infiltration in tumors was analyzed by immune infiltration correlation analysis,the association between CEACAM1 and multiple immune checkpoints was analyzed by single co-expression analysis,and the pathway associated with CEACAM1 was identified by gene enrichment analysis(GSEA).Results The expression of CEACAM1 in tumor tissues was higher than that in adjacent tissues,with statistical significance(P<0.05).Immunohistochemical also showed that the fluorescence intensity of CEACAM1 in tumor tissues was stronger than that in the normal lung tissues.KM survival curve showed that the OS of the CEACAM1 high expression group was lower than that of the low expression group,especially in stage I and II LUAD patients,with statistical significance(P<0.05).The correlation analysis of immune infiltration showed that CEACAM1 was positively correlated with the infiltration of T follicular helper cells(TFH),activated dendritic cells(aDC),T regulatory cells(Treg),T cells,Eosinophils,B cells,Th1 cells and immature dendritic cell(iDC)(P<0.05),of which TFH was the most significant.Co-expression analysis showed that CEACAM1 was positively correlated with SIGLEC15,TIGIT,CD274,HAVCR2,PDCD1,CTLA4 and LAG3,with statistical significance(P<0.05).GSEA illustrated that CEACAM1 was related to Rho GTPase signaling,M-phase mitosis,NABA secretion factors,DNA repair and metabolism of amino acids and derivatives in LUAD.Conclusion CEACAM1 may bec

关 键 词：癌胚抗原相关细胞黏附分子1 肺腺癌 免疫浸润 免疫检查点 信号通路 

分 类 号：R734.2[医药卫生—肿瘤]