Circ_0032821/miR-936/CEP55调控轴在胃癌组织中的表达及与临床特征之间的关系  

The expression of circ_0032821/miR-936/CEP55 regulatory axis in gastric cancer and its relationship with clinical features

作　　者：郑娴娴 高明[2] ZHENG Xianxian;GAO Ming(Department of Clinical Laboratory,South Binhu Hospital of Hefei First People's Hospital,Hefei,Anhui 230000,China;Department of Emergency Surgery,the Second Affiliated Hospital of Anhui Medical University,Hefei,Anhui 230000,China)

机构地区：[1]合肥市第一人民医院南区滨湖医院检验科,安徽合肥230000 [2]安徽医科大学第二附属医院急诊外科,安徽合肥230000

出　　处：《国际检验医学杂志》2023年第16期1993-1999,共7页International Journal of Laboratory Medicine

摘　　要：目的通过生物信息学技术构建胃癌相关circRNA的内源竞争性RNA(ceRNA)调控网络并探讨其临床意义。方法挖掘基因表达图谱(GEO)数据库中差异表达的环状RNA(circRNA)、微小RNA(miRNA)、信使RNA(mRNA),Cytoscpe软件构建circRNA、miRNA、mRNA之间的ceRNA调控网络,并对差异表达的mRNA进行基因本体论和京都基因与基因组百科全书数据库通路富集分析,最后利用癌症基因组图谱(TCGA)数据库的343个胃癌组织标本和30个正常组织标本的临床资料对ceRNA网络中的mRNA进行生存分析和表达验证,筛选其中重要的circRNA-miRNA-mRNA调控网络。在胃癌组织中对ceRNA的表达进行初步验证,并分析其与临床特征之间的关系。结果构建的circRNA-miRNA-mRNA调控网络中包含6个circRNA、4个miRNA和21个mRNA。根据TCGA数据库中胃癌患者临床资料分析和表达验证,ceRNA网络中CEP55和CCDC80与患者生存预后相关,CEP55在胃癌肿瘤标本中的表达显著高于正常组织标本中的表达,差异有统计学意义(P<0.05),最终筛选出circ_0032821-miR936-CEP55是胃癌的关键调控网络。经临床验证发现circ_0032821、miR-936及CEP55均在胃癌组织中差异表达,且circ_0032821的表达量与临床分期、淋巴结转移及浸润深度密切相关。结论circ_0032821-miR-936-CEP55调控轴在胃癌组织中差异表达,且circ_0032821表达与胃癌的进展相关。Objective To construct the ceRNA regulatory network of gastric cancer-related circRNAs by bioinformatics technology and explore its clinical significance.Methods The differentially expressed circRNAs,miRNAs,and mRNAs in the gene expression omnibus(GEO) were mined,and the Cytoscpe software was used to construct the ceRNA regulatory network of circRNA-miRNA-mRNA.The differentially expressed mRNAs in the GEO database were enriched by gene ontology and kyoto encyclopedia of genes and genomes.Finally,the clinical data of 343 gastric cancer tissue samples and 30 normal tissue samples in the cancer genome atlas(TCGA) database were used to perform survival analysis and expression verification of the mRNAs in the ceRNA network,and screen the important circRNA-miRNA-mRNA regulatory network.Preliminarily verify the expression of ceRNA in gastric cancer tissue,and analyze the relationship between ceRNA expression and clinical characteristics.Results The constructed circRNA-miRNA-mRNA regulatory network contained 6 circRNAs,4 miRNAs and 21 mRNAs.According to the clinical data analysis and expression verification of gastric cancer patients in the TCGA database,CEP55 and CCDC80 in the ceRNA network were related to the survival time of patients,and the expression of CEP55 in gastric cancer samples was significantly higher than that in normal tissue samples,and the difference was statistically significant(P<0.05).Finally,circ_0032821-miR-936-CEP55 was screened out as the key regulatory network for gastric cancer.Clinical verification showed that circ_0032821,mir-936 and CEP55 were differentially expressed in gastric cancer,and the expression of circ_0032821 was closely related to clinical stage,lymph node metastasis and depth of invasion.Conclusion Circ_0032821-miR-936-CEP55 regulatory axis is differentially expressed in gastric cancer.The expression of circ_0032821 is related to the progression of gastric cancer.

关 键 词：胃癌 生物信息学 circ_0032821 ceRNA调控网络 

分 类 号：R735.2[医药卫生—肿瘤]