Exploration on the mechanism of Radix Astragali-Caulis Spatholobi by Qi-invigorating and blood-activating combination for the treatment of atherosclerosis based on network pharmacology  

作　　者：Hai-Hua Lv Chen-Chen Huang Ying-Jun He Hong-Jie Liao Ting Zhao Suo-Yi Huang 

机构地区：[1]College of Clinical Medicine,YouJiang Medical University for Nationalities,Baise 533000,China [2]College of Pharmacy,YouJiang Medical University for Nationalities,Baise 533000,China [3]Key Laboratory of Guangxi’s College for the Study of Characteristic Medicine in Youjiang River Basin,Scientific Experiment Center of Youjiang Medical University for Nationalities,Baise 533000,China.

出　　处：《Drug Combination Therapy》2023年第3期1-8,共8页药物联合治疗

基　　金：Guangxi Key Research and Development Plan Project(Gui Ke AB18221095);China National Region Undergraduate Innovation and Entrepreneurship Training Program Funded Project(No.202110599016);Guangxi Zhuang Autonomous Region Undergraduate Innovation and Entrepreneurship Training Program Funded Project(No.S202210599105).

摘　　要：The objective of this study was to investigate the main active ingredients,potential targets,and possible mechanisms of action of the combination of Radix Astragali and Caulis Spatholobi for the treatment of atherosclerosis using network pharmacology.The study aimed to provide a reference basis for the development of new formulations and clinical use of Chinese medicine.The main components of Radix Astragali and Caulis Spatholobi were obtained from the TCMSP,BATMAN-TCM database,and literature reports.The targets corresponding to the main components were imported into the Uniprot database to standardize the names,and target information was supplemented with the Swiss Target Prediction database.Disease-related targets were obtained from DrugBank,OMIM,CTD,GeneCards,and DisGeNET online databases.Venn tools were used to obtain the potential targets of Radix Astragali and Caulis Spatholobi for the treatment of AS.The intersecting genes were imported into the STRING 11.5 database to construct protein-protein interaction network maps and analyze their interactions.Cytoscape 3.7.1 software was used to mine their core targets.GO function and KEGG signaling pathway enrichment analysis were performed using the DAVID v2023q1 database.The results were imported into the“Bioinformatics Cloud Platform”to generate enrichment bubble maps.Finally,the“component-target-pathway”diagram was constructed using Cytoscape 3.7.1 software.The study found that 78 major active ingredients and 527 potential targets were obtained from Radix Astragali and Caulis Spatholobi.The main active components of the two in combination for the treatment of AS are quercetin,stigmasterol,kaempferol,luteolin,formononetin,etc.The key targets involve CDKN1A,E2F1,CDK4,CDK2,CDK1,RB1,TP53,CDKN1B,IL6,JUN,etc.The main pathways involved the AGE-RAGE signaling pathway in diabetic complications,cancer pathway,etc.The biological processes involved include positive regulation of gene expression,negative regulation of apoptotic process,etc.The study initially verifi

关 键 词：network pharmacology Qi-invigorating blood-activating ATHEROSCLEROSIS 

分 类 号：R28[医药卫生—中药学]